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Hetastarch reduces neuronal cell death caused by oxidative stress

Title
Hetastarch reduces neuronal cell death caused by oxidative stress
Author
고성호
Keywords
Free radical; Hetastarch; Hydrogen peroxide; Ischemic stroke; Neuroprotection; Oxidative stress; PC12 cells
Issue Date
2012-02
Publisher
John Wiley & Sons, Ltd
Citation
Drug Development Research, Vol.73, No.1 [2012], p35-42
Abstract
We investigated the effect of hetastarch, used for the treatment of acute ischemic stroke, on neuronal cell damage by oxidative stress, a main pathogenic mechanism in ischemic stroke. Neuronally differentiated PC12 cells (nPC12 cells) were treated with varying concentrations of hetastarch and hydrogen peroxide (H2O2), and their viability was measured with a 3,(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay and trypan blue staining. The effect of hetastarch on free radical production by H2O2 was evaluated using the fluorescent probe 2′,′‐dichlorodihydrofluorescein diacetate (DCFH‐DA) and by quantifying the amount of 2,5‐ and 2,3‐dihydroxybenzoic acid (DHBA). Additionally, the expression levels of BAX, Bid, Bcl‐2, Bcl‐xL, cytosolic cytochrome c, and cleaved caspase‐3 were examined using Western blot analysis. Following exposure to 100 µM H2O2, the viability of nPC12 cells significantly decreased; however, cell viability increased with hetastarch treatment. Free radical production related to H2O2 exposure was significantly reduced after 100 µM hetastarch treatment. The expression levels of BAX, Bid, cytosolic cytochrome c, and activated caspase‐3 were reduced, whereas Bcl‐2 and Bcl‐xL levels increased in H2O2‐injured nPC12 cells treated with 100 µM hetastarch, as compared with nPC12 cells that were treated with only 100 µM H2O2. These results demonstrate that hetastarch can reduce oxidative stress‐induced neuronal cell death. Drug Dev Res 73: 35–42, 2012.
URI
http://onlinelibrary.wiley.com/doi/abs/10.1002/ddr.20447http://hdl.handle.net/20.500.11754/67509
ISSN
0272-4391
DOI
10.1002/ddr.20447
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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