Detection of Clostridium difficile toxin A/B genes by multiplex real-time PCR for the diagnosis of C. difficile infection

Title
Detection of Clostridium difficile toxin A/B genes by multiplex real-time PCR for the diagnosis of C. difficile infection
Other Titles
B genes by multiplex real-time PCR for the diagnosis of C. difficile infection
Author
이양순
Keywords
Bacterial Proteins; analysis; genetics; Bacterial Toxins; Bacteriological Techniques; methods; Clostridium Infections; diagnosis, microbiology; Clostridium difficile; isolation & purification; Enterotoxins; Feces
Issue Date
2012-02
Publisher
Elsevier
Citation
JOURNAL OF MEDICAL MICROBIOLOGY, 2012, 61(2), P.274p ~ 281
Abstract
Toxigenic Clostridium difficile culture is considered to be the standard diagnostic method for the detection of C. difficile infection (CDI). Culture methods are time-consuming and although enzyme immunoassay is rapid and easy to use, it has low sensitivity. In the present study, the AdvanSure CD real-time (RT)-PCR kit (LG Life Sciences) was evaluated for its ability to detect C. difficile toxin A (tcdA) and B (tcdB) genes, simultaneously. A total of 127 fresh diarrhoeal stool specimens, submitted to the clinical microbiology laboratory for C. difficile culture, were tested. C. difficile toxins and toxin genes were detected with a VIDAS C. difficile A&B (VIDAS-CDAB) enzyme-linked fluorescent immunoassay (ELFA) and the AdvanSure RT-PCR kit, respectively, according to the manufacturers’ instructions. Their performance was compared with a standard toxigenic culture method as a reference. The sensitivity, specificity and positive and negative predictive values using the AdvanSure RT-PCR kit were 100 %, 98.3 %, 84.6 % and 100 %, respectively, while those of the VIDAS-CDAB system were 63.6 %, 100 %, 100 % and 96.6 %, respectively. Four tcdA +/tcdB + strains of C. difficile were detected with the AdvanSure RT-PCR kit, which offers comparable sensitivity and specificity to the reference method with a turnaround time of ~3 hours.
URI
http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.035618-0http://hdl.handle.net/20.500.11754/67502
ISSN
0022-2615
DOI
10.1099/jmm.0.035618-0
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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