Novel Cyanine Dyes with Vinylsulfone Group for Labeling Biomolecules
- Title
- Novel Cyanine Dyes with Vinylsulfone Group for Labeling Biomolecules
- Author
- 이기정
- Keywords
- N-HYDROXYSUCCINIMIDE ESTERS; REAGENTS; KINETICS; PROBES; DNA
- Issue Date
- 2012-03
- Publisher
- ACS AMERICAN CHEMICAL SOCIETY
- Citation
- BIOCONJUGATE CHEMISTRY, Vol.23, No.3 [2012], p350-362
- Abstract
- Novel vinylsulfone cyanine dyes (em. 550-850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.
- URI
- https://pubs.acs.org/doi/10.1021/bc200232dhttp://hdl.handle.net/20.500.11754/67492
- ISSN
- 1043-1802
- DOI
- 10.1021/bc200232d
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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