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Amphiphilic peptides with arginine and valine residues as siRNA carriers

Title
Amphiphilic peptides with arginine and valine residues as siRNA carriers
Author
이민형
Keywords
CYTOTOXICITY; GENE DELIVERY; GENE THERAPY; MICELLE; PEPTIDE
Issue Date
2012-02
Publisher
John Wiley & Sons, Ltd
Citation
JOURNAL OF CELLULAR BIOCHEMISTRY, 2012, 113(2), P.619-628
Abstract
An efficient and safe delivery carrier is required for the therapeutic application of siRNA. In this research, amphiphilic peptides with arginine and valine residues were evaluated as siRNA carriers. The peptides were composed of 14 arginine-blocks and 6 valine-blocks. In the aqueous solution, the argininevaline peptides (RV peptides) formed micelles with hydrophobic cores comprised of a valine block and a cationic surface comprised of an arginine block. In a gel retardation assay, the RV peptides completely retarded siRNA at a 1:10 weight ratio (siRNA:peptide). A heparin competition assay suggested that the RV peptides formed more stable complexes with siRNA than they did with polyethylenimine (25?kDa, PEI25k). In an in vitro silencing assay, a dual luciferase expression (Renilla and firefly luciferases) vector, psiCHECK2, was co-transfected into human embryonic kidney 293 cells with Renilla-siRNA using the RV peptides. The specific silencing effect of Renilla luciferase was analyzed in reference to firefly luciferase. The results showed that the R3V6 peptide was more efficient than the R1V6, R2V6, and R4V6 peptides in silencing Renilla luciferase. In the flow cytometry and in vitro silencing studies, the R3V6 peptide delivered Renila-siRNA as efficiently as PEI25k. The siVEGF/R3V6 peptide also reduced endogenous vascular endothelial growth factor (VEGF) expression in CT27 cells as efficiently as PEI25k. A cytotoxicity assay showed that RV peptides did not cause any cytotoxicity. Therefore, RV peptides may be useful for the development of a safe and efficient delivery carrier of siRNA. J. Cell. Biochem. 113: 619628, 2012. (C) 2011 Wiley Periodicals, Inc.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.23389http://hdl.handle.net/20.500.11754/67461
ISSN
0730-2312
DOI
10.1002/jcb.23389
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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