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Circulating Gut-Homing (alpha(4)beta(+)(7)) Plasmablast Responses against Shigella Surface Protein Antigens among Hospitalized Patients with Diarrhea

Title
Circulating Gut-Homing (alpha(4)beta(+)(7)) Plasmablast Responses against Shigella Surface Protein Antigens among Hospitalized Patients with Diarrhea
Author
김동욱
Keywords
ANTIBODY-FORMING-CELLS; MUCOSAL IMMUNE-RESPONSES; VACCINE DEVELOPMENT; DYSENTERIC PATIENTS; SECRETING CELLS; IMMUNOGENICITY; INFECTION; SONNEI; IMMUNIZATIONS
Issue Date
2016-07
Publisher
AMER SOC MICROBIOLOGY
Citation
CLINICAL AND VACCINE IMMUNOLOGY, v. 23, No. 7, Page. 610-617
Abstract
Developing countries are burdened with Shigella diarrhea. Understanding mucosal immune responses associated with natural Shigella infection is important to identify potential correlates of protection and, as such, to design effective vaccines. We performed a comparative analysis of circulating mucosal plasmablasts producing specific antibodies against highly conserved invasive plasmid antigens (IpaC, IpaD20, and IpaD120) and two recently identified surface protein antigens, pan-Shigella surface protein antigen 1 (PSSP1) and PSSP2, common to all virulent Shigella strains. We examined blood and stool specimens from 37 diarrheal patients admitted to the Infectious Diseases Beliaghata General Hospital, Kolkata, India. The etiological agent of diarrhea was investigated in stool specimens by microbiological methods and real-time PCR. Gut-homing (alpha(4)beta(+)(7)) antibody secreting cells (ASCs) were isolated from patient blood by means of combined magnetic cell sorting and two-color enzymelinked immunosorbent spot (ELISPOT) assay. Overall, 57% (21 of 37) and 65% (24 of 37) of the patients were positive for Shigella infection by microbiological and real-time PCR assays, respectively. The frequency of alpha(4)beta(+)(7) IgG ASC responders against Ipas was higher than that observed against PSSP1 or PSSP2, regardless of the Shigella serotype isolated from these patients. Thus, alpha(4)beta(+)(7) ASC responses to Ipas may be considered an indirect marker of Shigella infection. The apparent weakness of ASC responses to PSSP1 is consistent with the lack of cross-protection induced by natural Shigella infection. The finding that ASC responses to IpaD develop in patients with recent-onset shigellosis indicates that such responses may not be protective or may wane too rapidly and/or be of insufficient magnitude.
URI
http://cvi.asm.org/content/23/7/610.full.pdfhttp://hdl.handle.net/20.500.11754/67273
ISSN
1556-6811
DOI
10.1128/CVI.00205-16
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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