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Tianma modulates proteins with various neuro-regenerative modalities in differentiated human neuronal SH-SY5Y cells

Title
Tianma modulates proteins with various neuro-regenerative modalities in differentiated human neuronal SH-SY5Y cells
Author
Klaus Heese
Keywords
Tianma; Neuron; Herb; TCM; iTRAQ; Neurodegeneration
Issue Date
2012-06
Publisher
Elsevier Science LTD
Citation
Neurochemistry International, 2012, 60(8), P.827-836
Abstract
Tianma (Rhizome gastrodiae) is the dried rhizome of the plant Gastrodia elate Blume (Orchidaceae family). As a medicinal herb in traditional Chinese medicine (TCM) its functions are to control convulsions, pain, headache, dizziness, vertigo, seizure, epilepsy and others. In addition, tianma is frequently used for the treatment of neurodegenerative disorders though the mechanism of action is widely unknown. Accordingly, this study was designed to examine the effects of tianma on the proteome metabolism in differentiated human neuronal SH-SY5Y cells to explore its specific effects on neuronal signaling pathways. Using an iTRAQ (isobaric tags for relative and absolute quantitation)-based proteomics research approach, we identified 2390 modulated proteins, out of which 406 were found to be altered by tianma in differentiated human neuronal SH-SY5Y cells. Based on the observed data, we hypothesize that tianma promotes neuro-regenerative signaling cascades by controlling chaperone/proteasomal degradation pathways (e.g. CALR, FKBP3/4, HSP70/90) and mobilizing neuro-protective genes (such as AIP5) as well as modulating other proteins (RTN1/4, NCAM, PACSIN2, and PDLIM1/5) with various regenerative modalities and capacities related to neuro-synaptic plasticity. (C) 2012 Elsevier Ltd. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0197018612001143?via%3Dihubhttp://hdl.handle.net/20.500.11754/67242
ISSN
0197-0186; 1872-9754
DOI
10.1016/j.neuint.2012.03.012
Appears in Collections:
GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > ETC
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