Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-04-16T00:51:27Z | - |
dc.date.available | 2018-04-16T00:51:27Z | - |
dc.date.issued | 2012-01 | - |
dc.identifier.citation | Journal of Controlled Release, Vol.157, No.2 [2012], p243-248 | en_US |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0168365911008571?via%3Dihub | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/67191 | - |
dc.description.abstract | Glucagon-like peptide (GLP-1) encoding dual plasmid (pDNA) system (TSTA (SP-GLP-1)) which is composed of pβ-Gal4-p65 and pUAS-SP-GLP-1 was constructed to improve the production and secretion of expressed GLP-1 by combining the advantages of signal peptide (SP) and two-step transcription amplification (TSTA) system. Its potential for GLP-1 gene delivery system was investigated with employment of arginine-grafted bioreducible polymer (ABP) as a gene carrier. Their polyplexes have about 140nm-sizes and 20mV Zeta-potential values. ABP showed no cytotoxicity contrary to PEI25k. It was found in RT-PCR experiments that TSTA-SP pDNA systems showed increased GLP-1 gene transcription level in comparison with mono pDNA system (pβ-GLP-1). It was also observed in GLP-1 ELISA that GLP-1 secretion level of TSTA (SP-GLP-1) pDNA system was 2.7–3.4 times higher than those of pβ-GLP-1 and 1.5–1.7 times than TSTA (GLP-1). Additionally, 2.5–3.5 folds increased level of GLP-1 secretion was found in ABP gene carrier system in comparison with PEI25k. When transfection medium containing secreted GLP-1 was transferred to NIT-1 insulinoma cells, the highest secretion level of insulin was induced in ABP/TSTA (SP-GLP-1) polyplex medium-treated cells. Therefore, this novel system could be utilized as a safe and efficient GLP-1 gene delivery system for type 2 diabetes therapy. | en_US |
dc.description.sponsorship | This work was supported by NIH grant (DK 077703) and Research fund (WCU 200900000000024 and 2010K80183) of the Ministry of Education, Science and Technology, Korea. We thank Prof. You Han Bae for the generous permission of Zetasizer. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science B.V., Amsterdam. | en_US |
dc.subject | Gene delivery | en_US |
dc.subject | Glucagon-like peptide-1 | en_US |
dc.subject | Two-step transcription amplification | en_US |
dc.subject | Secretion signal peptide | en_US |
dc.subject | Arginine-grafted bioreducible polymer | en_US |
dc.title | Efficient GLP-1 gene delivery using two-step transcription amplification plasmid system with a secretion signal peptide and arginine-grafted bioreducible polymer | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 157 | - |
dc.identifier.doi | 10.1016/j.jconrel.2011.09.072 | - |
dc.relation.page | 243-248 | - |
dc.relation.journal | JOURNAL OF CONTROLLED RELEASE | - |
dc.contributor.googleauthor | Kim, Tae-il | - |
dc.contributor.googleauthor | Lee, Min-Hyung | - |
dc.contributor.googleauthor | Kim, Sung-Wan | - |
dc.relation.code | 2012204927 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
dc.identifier.researcherID | 7409120793 | - |
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