A Microfluidic Approach to Encapsulate Living Cells in Uniform Alginate Hydrogel Microparticles
- Title
- A Microfluidic Approach to Encapsulate Living Cells in Uniform Alginate Hydrogel Microparticles
- Author
- 김진웅
- Keywords
- alginate hydrogels; cells; double emulsions; encapsulation; microfluidics
- Issue Date
- 2012-07
- Publisher
- John Wiley & Sons, Ltd
- Citation
- MACROMOLECULAR BIOSCIENCE, 2012, 12(7), P.946-951
- Abstract
- A microfluidic technique is described to encapsulate living cells in alginate hydrogel microparticles generated from monodisperse double-emulsion templates. A microcapillary device is used to fabricate double emulsion templates composed of an alginate drop surrounded by a mineral oil shell. Hydrogel formation begins when the alginate drop separates from the mineral oil shell and comes into contact with Ca(2+) ions in the continuous phase. Alginate hydrogel microparticles with diameters ranging from 60 to 230 µm are obtained. 65% of the cells encapsulated in the alginate microparticles were viable after one week. The technique provides a useful means to encapsulate the living cells in monodisperse hydrogel microparticles.
- URI
- https://onlinelibrary.wiley.com/doi/abs/10.1002/mabi.201100351http://hdl.handle.net/20.500.11754/67158
- ISSN
- 1616-5187
- DOI
- 10.1002/mabi.201100351
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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