A Microfluidic Approach to Encapsulate Living Cells in Uniform Alginate Hydrogel Microparticles

Title
A Microfluidic Approach to Encapsulate Living Cells in Uniform Alginate Hydrogel Microparticles
Author
김진웅
Keywords
alginate hydrogels; cells; double emulsions; encapsulation; microfluidics
Issue Date
2012-07
Publisher
John Wiley & Sons, Ltd
Citation
MACROMOLECULAR BIOSCIENCE, 2012, 12(7), P.946-951
Abstract
A microfluidic technique is described to encapsulate living cells in alginate hydrogel microparticles generated from monodisperse double-emulsion templates. A microcapillary device is used to fabricate double emulsion templates composed of an alginate drop surrounded by a mineral oil shell. Hydrogel formation begins when the alginate drop separates from the mineral oil shell and comes into contact with Ca(2+) ions in the continuous phase. Alginate hydrogel microparticles with diameters ranging from 60 to 230 µm are obtained. 65% of the cells encapsulated in the alginate microparticles were viable after one week. The technique provides a useful means to encapsulate the living cells in monodisperse hydrogel microparticles.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/mabi.201100351http://hdl.handle.net/20.500.11754/67158
ISSN
1616-5187
DOI
10.1002/mabi.201100351
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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