Expression of amnionless in mouse testes and Leydig cells

Title
Expression of amnionless in mouse testes and Leydig cells
Authors
오영석
Keywords
Amnionless; germ cells; Leydig cells; mouse testes; vitamin B12
Issue Date
2012-05
Publisher
Blackwell Publishing Ltd
Citation
Andrologia, Vol.44, No.S1 [2012], p383-389
Abstract
Vitamin B12 (cobalamin) deficiency results in atrophy of seminiferous tubules and aplasia of spermatozoa and spermatid. The transmembrane protein amnionless (AMN) directs endocytosis of cubilin with its ligand, contributing to intrinsic factor-vitamin B12 absorption. To understand vitamin B12 transport in testis, we analysed AMN expression in developing mouse testes and in Leydig cells and speculated the possible role of AMN in testis. In testes, Amn mRNA levels were low until 14 days post partum ( pp) and markedly increased from puberty onwards. In the interstitium, Amn mRNA levels were low at 14 days pp and increased at puberty (28 days pp) together with 3-beta-hydroxysteroid dehydrogenase type 6 mRNA. Strong AMN immunoreactivity was observed in early spermatocytes from 7 days pp, suggesting that AMN participates in meiosis. In Leydig cells, AMN was not observed until 14 days pp but was strongly expressed after 28 days pp, suggesting a positive relationship between AMN expression and functional differentiation of adult Leydig cells. Together, AMN may participate in meiosis in early spermatocytes and in functional differentiation of adult Leydig cells through the mediation of vitamin B12 transport in the mouse testes. This is the first report on AMN expression in the germ cells and soma of mammalian testes.
URI
http://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0272.2011.01195.xhttp://hdl.handle.net/20.500.11754/67046
ISSN
0303-4569
DOI
http://dx.doi.org/10.1111/j.1439-0272.2011.01195.x
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RESEARCH INSTITUTE[S](부설연구소) > THE RESEARCH INSTITUTE FOR NATURAL SCIENCES(자연과학연구소) > Articles
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