Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 주재범 | - |
dc.date.accessioned | 2018-04-15T14:35:19Z | - |
dc.date.available | 2018-04-15T14:35:19Z | - |
dc.date.issued | 2012-05 | - |
dc.identifier.citation | Journal of Molecular Structure, Vol.1023, No.- [2012], p197-203 | en_US |
dc.identifier.issn | 0022-2860 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0022286012003420?via%3Dihub | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/67007 | - |
dc.description.abstract | We report a highly sensitive surface-enhanced Raman scattering (SERS)-based immunoassay platform for the multiplex detection of biomarkers. For this purpose, a gold-patterned microarray chip has been fabricated and used as a SERS detection template. Here, a typical sandwich immunocomplex protocol was adopted. Monoclonal antibodies were immobilized on gold patterned substrates, and then antigen solutions and polyclonal antibody-conjugated hollow gold nanospheres (HGNs) were sequentially added for the formation of sandwich immunocomplexes. Antigen biomarkers can be quantitatively assayed by monitoring the intensity change of a characteristic SERS peak of a reporter molecule adsorbed on the surfaces of HGNs. Under optimized assay conditions, the limits of detections (LODs) were determined to be 10fg/mL for human IgG and 10–100fg/mL for rabbit IgG. In addition, the SERS-based immunoassay technique can be applied in a wider dynamic concentration range with a good sensitivity compared to ELISA. The proposed method fulfills the current needs of high sensitivity and selectivity which are essential for the clinical diagnosis of a disease. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (Grant Numbers R11-2008-0061852 and K20904000004-09A0500-00410) and by the Agency for Defense Development through Chemical and Biological Defense Research Center. URC acknowledges financial support from the Higher Education Commission (HEC) Program of the government of Pakistan. This work was also partially supported by the Ministry of Knowledge Economy (MKE) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science B.V., Amsterdam. | en_US |
dc.subject | Microarray chip | en_US |
dc.subject | Hollow gold nanospheres | en_US |
dc.subject | Surface-enhanced Raman scattering | en_US |
dc.subject | Multiplex assayImmunoassay | en_US |
dc.subject | ELISA | en_US |
dc.title | SERS-based multiple biomarker detection using a gold-patterned microarray chip | en_US |
dc.type | Article | en_US |
dc.relation.volume | 1023 | - |
dc.identifier.doi | 10.1016/j.molstruc.2012.04.031 | - |
dc.relation.page | 197-203 | - |
dc.relation.journal | JOURNAL OF MOLECULAR STRUCTURE | - |
dc.contributor.googleauthor | Kim, I. | - |
dc.contributor.googleauthor | Junejo, I. u. | - |
dc.contributor.googleauthor | Lee, M. | - |
dc.contributor.googleauthor | Lee, S. | - |
dc.contributor.googleauthor | Lee, E. K. | - |
dc.contributor.googleauthor | Chang, S. I. | - |
dc.contributor.googleauthor | Choo, J. | - |
dc.relation.code | 2012205457 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jbchoo | - |
dc.identifier.researcherID | 7006799883 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.