SERS-based multiple biomarker detection using a gold-patterned microarray chip

Title
SERS-based multiple biomarker detection using a gold-patterned microarray chip
Author
주재범
Keywords
Microarray chip; Hollow gold nanospheres; Surface-enhanced Raman scattering; Multiplex assayImmunoassay; ELISA
Issue Date
2012-05
Publisher
Elsevier Science B.V., Amsterdam.
Citation
Journal of Molecular Structure, Vol.1023, No.- [2012], p197-203
Abstract
We report a highly sensitive surface-enhanced Raman scattering (SERS)-based immunoassay platform for the multiplex detection of biomarkers. For this purpose, a gold-patterned microarray chip has been fabricated and used as a SERS detection template. Here, a typical sandwich immunocomplex protocol was adopted. Monoclonal antibodies were immobilized on gold patterned substrates, and then antigen solutions and polyclonal antibody-conjugated hollow gold nanospheres (HGNs) were sequentially added for the formation of sandwich immunocomplexes. Antigen biomarkers can be quantitatively assayed by monitoring the intensity change of a characteristic SERS peak of a reporter molecule adsorbed on the surfaces of HGNs. Under optimized assay conditions, the limits of detections (LODs) were determined to be 10fg/mL for human IgG and 10–100fg/mL for rabbit IgG. In addition, the SERS-based immunoassay technique can be applied in a wider dynamic concentration range with a good sensitivity compared to ELISA. The proposed method fulfills the current needs of high sensitivity and selectivity which are essential for the clinical diagnosis of a disease.
URI
http://www.sciencedirect.com/science/article/pii/S0022286012003420?via%3Dihubhttp://hdl.handle.net/20.500.11754/67007
ISSN
0022-2860
DOI
10.1016/j.molstruc.2012.04.031
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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