37 0

Molecularly Engineered Islet Cell Clusters for Diabetes Mellitus Treatment

Title
Molecularly Engineered Islet Cell Clusters for Diabetes Mellitus Treatment
Author
이민형
Keywords
Islet cell clusters (ICCs); Exendin-4; Polyethylene glycol; Transplantation
Issue Date
2012-05
Publisher
COGNIZANT COMMUNICATION CORPORATION
Citation
CELL TRANSPLANTATION, Vol.21, No.8 [2012], p1775-p1789
Abstract
Pancreatic islet transplantation is a promising method for curing diabetes mellitus. We proposed in this study a molecularly engineered islet cell clusters (ICCs) that could overcome problems posed by islet transplantation circumstances and host's immune reactions. A gene containing highly releasable exendin-4, an insulinotropic protein, was delivered into single islet cells to enhance glucose sensitivity; thereafter, the cells were reaggregated into small size ICCs. Then the surface of ICCs was modified with biocompatible poly(ethylene glycol)lipid (PEG) (C18) for preventing immune reactions. The regimen of ICCs with low doses of anti-CD154 mAb and tacrolimus could effectively maintain the normal glucose level in diabetic mice. This molecularly engineered PEG-Sp-Ex-4 ICC regimen prevented cell death in transplantation site, partly through improving the regulation of glucose metabolism and by preventing hypoxia- and immune response-induced apoptosis. Application of this remedy is also potentially far-reaching; one would be to help overcome islet supply shortage due to the limited availability of pancreas donors and reduce the immunosuppressant regimens to eliminate their adverse effects.
URI
http://journals.sagepub.com/doi/10.3727/096368912X640628http://hdl.handle.net/20.500.11754/66943
ISSN
0963-6897
DOI
10.3727/096368912X640628
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE