64 0

T Cell-Specific siRNA Delivery Using Antibody-Conjugated Chitosan Nanoparticles

Title
T Cell-Specific siRNA Delivery Using Antibody-Conjugated Chitosan Nanoparticles
Author
임태연
Keywords
GENE DELIVERY; RNA INTERFERENCE; DNA NANOPARTICLES; HIV-1 INFECTION; SYSTEMS; APOPTOSIS; THERAPY; CARRIER
Issue Date
2012-05
Publisher
ACS AMERICAN CHEMICAL SOCIETY
Citation
BIOCONJUGATE CHEMISTRY, Vol.23, No.6 [2012], p1174-1180
Abstract
The intracellular delivery of small interfering RNA (siRNA) plays a key role in RNA interference (RNA and provides an emerging technique to treat various diseases, including infectious diseases. Chitosan has frequently been used in gene delivery applications, including siRNA delivery. However, studies regarding the modification of chitosan with antibodies specifically targeting T cells are lacking. We hypothesized that chitosan nanoparticles modified with T cell-specific antibodies would be useful for delivering siRNA to T cells. CD7-specific single-chain antibody (scFvCD7) was chemically conjugated to chitosan by carbodiimide chemistry, and nanoparticles were prepared by a complex coacervation method in the presence of siRNA. The mean diameter and zeta potential of the scFvCD7-chitosan/siRNA nanoparticles were approximately 320 nm and +17 mV, respectively, and were not significantly influenced by the coupling of antibody to chitosan. The cellular association of antibody-conjugated nanoparticles to CD4+ T cell lines as well as gene silencing efficiency in the cells was significantly improved compared to nonmodified chitosan nanoparticles. This approach to introducing T cell-specific antibody to chitosan nanoparticles may find useful applications for the treatment of various infectious diseases.
URI
https://pubs.acs.org/doi/10.1021/bc2006219http://hdl.handle.net/20.500.11754/66933
ISSN
1043-1802
DOI
10.1021/bc2006219
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE