Global Gene Response in Saccharomyces cerevisiae Exposed to Silver Nanoparticles

Title
Global Gene Response in Saccharomyces cerevisiae Exposed to Silver Nanoparticles
Author
상병인
Keywords
Microarray; Nanotoxicity; Silver nanoparticles; Transcriptome analysis; Yeast genome analysis
Issue Date
2011-08
Publisher
Humana Press INC
Citation
Applied Biochemistry and Biotechnology, 2011, 164(8), P.1278-1291
Abstract
Silver nanoparticles (AgNPs), exhibiting a broad size range and morphologies with highly reactive facets, which are widely applicable in real-life but not fully verified for biosafety and ecotoxicity, were subjected to report transcriptome profile in yeast Saccharomyces cerevisiae. A large number of genes accounted for similar to 3% and similar to 5% of the genome affected by AgNPs and Ag-ions, respectively. Principal component and cluster analysis suggest that the different physical forms of Ag were the major cause in differential expression profile. Among 90 genes affected by both AgNPs and Ag-ions, metalloprotein mediating high resistance to copper (CUP1-1 and CUP1-2) were strongly induced by AgNPs (similar to 45-folds) and Ag-ions (similar to 22-folds), respectively. A total of 17 genes, responsive to chemical stimuli, stress, and transport processes, were differentially induced by AgNPs. The differential expression was also seen with Ag-ions that affected 73 up- and 161 down-regulating genes, and most of these were involved in ion transport and homeostasis. This study provides new information on the knowledge for impact of nanoparticles on living microorganisms that can be extended to other nanoparticles.
URI
https://link.springer.com/article/10.1007%2Fs12010-011-9212-4http://hdl.handle.net/20.500.11754/66806
ISSN
0273-2289; 1559-0291
DOI
10.1007/s12010-011-9212-4
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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