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Differential Physiological Roles of ESCRT Complexes in Caenorhabditis elegans

Title
Differential Physiological Roles of ESCRT Complexes in Caenorhabditis elegans
Author
안주홍
Keywords
C. elegans; endocytosis; ESCRT; protein degradation; yolk protein
Issue Date
2011-06
Publisher
한국분자세포생물학회
Citation
MOLECULES AND CELLS, 2011, 31(6), P.585-592
Abstract
Endosomal sorting complex required for transport (ESCRT) complexes are involved in endosomal trafficking to the lysosome, cytokinesis, and viral budding. Extensive genetic, biochemical, and structural studies on the ESCRT system have been carried out in yeast and mammalian systems. However, the question of how the ESCRT system functions at the whole organism level has not been fully explored. In C. elegans, we performed RNAi experiments to knock-down gene expression of components of the ESCRT system and profiled their effects on protein degradation and endocytosis of YP170, a yolk protein. Targeted RNAi knock-down of ESCRT-I (tsg-101 and vps-28) and ESCRT-III (vps-24, and vps-32.2) components interfered with protein degradation while knock-down of ESCRT-II (vps-25 and vps-36) and ESCRT-III (vps-20 and vps-24) components hampered endocytosis. In contrast, the knockdown of vps-37, another ESCRT-I component, showed no defect in either YP170 uptake or degradation. Depletion of at least one component from each complex — ESCRT-0 (hgrs-1), ESCRT-I (tsg-101, vps-28, and vps-37), ESCRT-II (vps-36), ESCRT-III (vps-24), and Vps4 (vps-4) — resulted in abnormal distribution of embryos in the uterus of worms, possibly due to abnormal ovulation, fertilization, and egglaying. These results suggest differential physiological roles of ESCRT-0, -I, -II, and -III complexes in the context of the whole organism, C. elegans.
URI
https://link.springer.com/article/10.1007%2Fs10059-011-1045-zhttp://hdl.handle.net/20.500.11754/66693
ISSN
1016-8478
DOI
10.1007/s10059-011-1045-z
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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