Preparation of Silica-Encapsulates Hollow Gold Nanosphere Tags Using layer-by-Layer Method for Multiplex SERS Detection

Title
Preparation of Silica-Encapsulates Hollow Gold Nanosphere Tags Using layer-by-Layer Method for Multiplex SERS Detection
Author
주재범
Keywords
NANOPARTICLE PROBES; SPECTROSCOPIC TAGS; POLY(ACRYLIC ACID); SERS; DNA; NANOPROBES; SPHERES; CELLS; AGGREGATION; IMMUNOASSAY
Issue Date
2011-06
Publisher
AMER CHEMICAL SOC
Citation
Langmuir : the ACS journal of surfaces and colloids, 2011, 27(16), P.10228~10261, 6P.
Abstract
The use of silica shells offers many advantages in surface-enhanced Raman scattering (SERS)-based biological sensing applications due to their optical transparency, remarkable stability in environmental media, and improved biocompatibility. Here, we report a novel layer-by-layer method for the preparation of silica-hollow gold nanosphere (HGN) SERS tags. Poly(acrylic acid) was used to stabilize Raman reporter-tagged HGNs prior to the adsorption of a coupling agent, after which a silica shell was deposited onto the particle surface using Stöber’s method. Importantly, competitive adsorption of the Raman reporter molecules and coupling agents, which results in unbalanced loading of reporter molecules on individual nanoparticles, was avoided using this method. As a result, the loading density of reporter molecules could be maximized. In addition, HGNs exhibited strong enhancement effects from the individual particles because of their ability to localize the surface electromagnetic fields through pinholes in the hollow particle structures. The proposed layer-by-layer silica-encapsulated HGN tags showed strong SERS signals as well as excellent multiplexing capabilities.
URI
https://pubs.acs.org/doi/abs/10.1021/la201739nhttp://hdl.handle.net/20.500.11754/66655
ISSN
0743-7463
DOI
10.1021/la201739n
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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