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dc.contributor.author이수재-
dc.date.accessioned2018-04-15T06:51:39Z-
dc.date.available2018-04-15T06:51:39Z-
dc.date.issued2011-06-
dc.identifier.citationNeurotoxicology December 2011 32(6):879-887en_US
dc.identifier.issn0161-813X-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0161813X11000982-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/66585-
dc.description.abstractThe neurotoxicity of L-3,4-dihydroxyphenylalanine (L-DOPA), one of the most important drugs for the treatment of Parkinson's disease, still remains controversial, although much more data on L-DOPA neurotoxicity have been presented. Considering the well known neuroprotective effects of erythropoietin (EPO), the inhibitory effects of EPO on L-DOPA neurotoxicity need to be evaluated. Neuronally differentiated PC12 (nPC12) cells were treated with different concentrations of L-DOPA and/or EPO for 24 h. Cell viability was evaluated using trypan blue, 4',6-diamidino-2-phenylindole (DAPI) and TUNEL staining, and cell counting. Free radicals and intracellular signaling protein levels were measured with 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) and Western blotting, respectively. L-DOPA reduced nPC12 cell viability at higher concentrations, but combined treatment with EPO and L-DOPA significantly restored cell viability. Free radicals and hydroxyl radical levels increased by L-DOPA were decreased after combined treatment of L-DOPA and EPO. Levels of survival-related intracellular signaling proteins decreased in nPC12 cells treated with 200 mu M L-DOPA but increased significantly in cells treated with 200 mu M L-DOPA and 5 mu M EPO. However, cleaved caspase-3, a death-related protein, increased in nPC12 cells treated with 200 mu M L-DOPA but decreased significantly in cells treated with 200 mu M L-DOPA and 5 mu M EPO. Pretreatment with LY294002, a phosphatidylinositol 3-kinase inhibitor, prior to combined treatment with EPO and L-DOPA almost completely blocked the protective effects of EPO. These results indicate that EPO can prevent L-DOPA neurotoxicity by activating the PI3K pathway as well as reducing oxidative stress. (C) 2011 Elsevier Inc. All rights reserved.en_US
dc.language.isoenen_US
dc.publisherElsevier Science B.V., Amsterdam.en_US
dc.subjectL-DOPAen_US
dc.subjectErythropoietinen_US
dc.subjectPhosphatidylinositol 3-kinaseen_US
dc.subjectNeurotoxicityen_US
dc.titleL-DOPA neurotoxicity is prevented by neuroprotective effects of erythropoietinen_US
dc.typeArticleen_US
dc.relation.no6-
dc.relation.volume32-
dc.identifier.doi10.1016/j.neuro.2011.05.009-
dc.relation.page879-887-
dc.relation.journalNEUROTOXICOLOGY-
dc.contributor.googleauthorPark, K. H.-
dc.contributor.googleauthorChoi, N. Y.-
dc.contributor.googleauthorKoh, S. H.-
dc.contributor.googleauthorPark, H. H.-
dc.contributor.googleauthorKim, Y. S.-
dc.contributor.googleauthorKim, M. J.-
dc.contributor.googleauthorLee, S. J.-
dc.contributor.googleauthorYu, H. J.-
dc.contributor.googleauthorLee, K. Y.-
dc.contributor.googleauthorLee, Y. J.-
dc.relation.code2011207057-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF LIFE SCIENCE-
dc.identifier.pidsj0420-
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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