Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-04-15T03:53:04Z | - |
dc.date.available | 2018-04-15T03:53:04Z | - |
dc.date.issued | 2011-03 | - |
dc.identifier.citation | TRANSPLANT INTERNATIONAL,권: 24 호: 3 페이지: 307-314 | en_US |
dc.identifier.issn | 0934-0874 | - |
dc.identifier.issn | 1432-2277 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-2277.2010.01194.x/ | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/66438 | - |
dc.description.abstract | P>For gene transfer strategies to improve islet engraftment, vascular endothelial growth factor (VEGF) expression should be regulated in a way that matches the transient nature of revascularization with simultaneously avoiding undesirable effects of overexpression. The aim of this study was to investigate the effects of hypoxia-inducible VEGF gene transfer using the RTP801 promoter on islet grafts. We implanted pSV-hVEGF transfected, pRTP801-hVEGF transfected or nontransfected mouse islets under the kidney capsule of streptozotocin-induced diabetic syngeneic mice. Human VEGF immunostaining of day 3 grafts revealed that the pRTP801-hVEGF transfected group had higher hVEGF expression compared with the pSV-hVEGF transfected group. BS-1 staining of day 3 grafts from the pRTP801-hVEGF transfected group showed the highest vascular density, which was comparable with day 6 grafts from the nontransfected group. In 360 islet equivalent (IEQ)-transplantation which reverted hyperglycemia in all mice, the area under the curve of glucose levels during intraperitoneal glucose tolerance test 7 weeks post-transplant was lower in mice transplanted with pRTP801-hVEGF transfected grafts compared with mice transplanted with nontransfected grafts. In 220 IEQ-transplantations, diabetic mice transplanted with pRTP801-hVEGF islets became normoglycemic more rapidly compared with mice transplanted with pSV-hVEGF or nontransfected islets, and diabetes reversal rate after 50 days was 90%, 68%, and 50%, respectively. In conclusion, our results indicate that regulated overexpression of hVEGF in a hypoxia-inducible manner enhances islet vascular engraftment and preserves islet function overtime in transplants. | en_US |
dc.description.sponsorship | This work was supported by a research grant from the Innovative Research Institute for Cell Therapy, Republic of Korea (A062260) to S.H.I., the Korea Research Foundation Grant (KRF-2008-331-E00038) from Korean Government (MOEHRD) to B.W.L. and the National Research Foundation of Korea from the Ministry of Education, Science and Technology (20090081874) to M.L. | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA | en_US |
dc.subject | hypoxia | en_US |
dc.subject | islet | en_US |
dc.subject | RTP801 | en_US |
dc.subject | transplantation | en_US |
dc.subject | vascular endothelial growth factor | en_US |
dc.title | Effect of hypoxia-inducible VEGF gene expression on revascularization and graft function in mouse islet transplantation | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 24 | - |
dc.identifier.doi | 10.1111/j.1432-2277.2010.01194.x | - |
dc.relation.page | 307-314 | - |
dc.relation.journal | TRANSPLANT INTERNATIONAL | - |
dc.contributor.googleauthor | Lee, Byung Wan | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.contributor.googleauthor | Chae, Hee Young | - |
dc.contributor.googleauthor | Lee, Sanghyun | - |
dc.contributor.googleauthor | Kang, Jun Goo | - |
dc.contributor.googleauthor | Kim, Chul Sik | - |
dc.contributor.googleauthor | Lee, Seong Jin | - |
dc.contributor.googleauthor | Yoo, Hyung Joon | - |
dc.contributor.googleauthor | Ihm, Sung-Hee | - |
dc.relation.code | 2011212793 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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