Proteomic Analysis of Terminalia chebula Extract-Dependent Changes in Human Lymphoblastic T Cell Protein Expression

Title
Proteomic Analysis of Terminalia chebula Extract-Dependent Changes in Human Lymphoblastic T Cell Protein Expression
Author
채영규
Keywords
gallic acid; Ingenuity Pathways Analysis; Jurkat cells; nuclear factor-kappa B; Terminalia chebula extract
Issue Date
2012-07
Publisher
Mary Ann Liebert, Inc.
Citation
Journal of Medicinal Food, 2012, 15(7), P.651-657, 7P.
Abstract
Terminalia chebula is a native plant from southern Asia to southwestern China that is used in traditional medicine for the treatment of malignant tumors and diabetes. This plant also has antibacterial and immunomodulatory properties. The present study assessed T. chebula extract-dependent protein expression changes in Jurkat cells. Matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry and Ingenuity Pathways Analysis (IPA) were performed to assess protein expression and networks, respectively. A comparative proteomic profile was determined in T. chebula extract (50 lg/mL)-treated and control cells; the expressions of b-tubulin, ring finger and CHY zinc finger domain containing 1, and insulinlike growth factor 1 receptor kinase were significantly down-regulated in T. chebula extract-treated Jurkat cells. Moreover, the molecular basis for the T. chebula extract-dependent protein expression changes in Jurkat cells was determined by IPA. Treatment with the T. chebula extract significantly inhibited nuclear factor-jB activity and affected the proteomic profile of Jurkat cells. The molecular network signatures and functional proteomics obtained in this study may facilitate the evaluation of potential antitumor therapeutic targets and elucidate the molecular mechanism of T. chebula extract-dependent effects in Jurkat cells.
URI
https://www.liebertpub.com/doi/abs/10.1089/jmf.2011.1998http://hdl.handle.net/20.500.11754/66403
ISSN
1096-620X
DOI
10.1089/jmf.2011.1998
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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