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Effects of L- and T-type Ca2+ channel blockers on spermatogenesis and steroidogenesis in the prepubertal mouse testis

Title
Effects of L- and T-type Ca2+ channel blockers on spermatogenesis and steroidogenesis in the prepubertal mouse testis
Author
계명찬
Keywords
Ca2+ channel blockers; Spermatogenesis; Steroidogenesis; Mouse prepubertal testis; RAT SERTOLI-CELLS; MALE GERM-CELLS; CALCIUM-CHANNELS; ANTIEPILEPTIC DRUGS; ACROSOME REACTION; MICE; SPERMIOGENESIS; HOMEOSTASIS; MECHANISMS; EXPRESSION
Issue Date
2011-01
Publisher
SPRINGER/PLENUM PUBLISHERS, 233 SPRING ST, NEW YORK, NY 10013 USA
Citation
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,Vol.28 No.1 [2011],23-30
Abstract
To assess the involvement of L-type and T-type Ca2+ channel blockers in inducing male infertility.Prepubertal male mice were fed Ca2+ channel blockers nifedipine and ethosuximide for 20 days at dosages below maximum tolerated dose (MTD) and assayed for gross morphological changes in the testis such as body weight, testis size and weight. Sperm and Leydig cell counting were conducted concomitantly with serum testosterone level measurement by radioimmunoassay (RIA) and StAR protein mRNA measurement by reverse transcription and polymerase chain reaction (RT-PCR).A chronic exposure to nifedipine or ethosuximide caused a significant reduction in body weight, testis size/weight and sperm production in a dose-dependent fashion associated with a spermatogenic arrest largely at the elongating spermatid stage. The number of Leydig cells, the serum testosterone level but not the luteinizing hormone level, and the content of StAR protein mRNA were also drastically reduced relative to the controls.Both T- and L-type Ca2+ channel blockers play an adverse role in normal spermatogenesis and steroidogenesis partly by blocking postmeiotic germ cell maturation and/or by abrogating StAR protein expression, contributing to male sterility. Therefore, any therapeutic application of Ca2+ channel blockers must be used with caution due to its potential adverse side effects on male infertility.
URI
https://link.springer.com/article/10.1007%2Fs10815-010-9480-xhttp://hdl.handle.net/20.500.11754/66068
ISSN
1058-0468; 1573-7330
DOI
10.1007/s10815-010-9480-x
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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