Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박현희 | - |
dc.date.accessioned | 2018-04-14T05:18:34Z | - |
dc.date.available | 2018-04-14T05:18:34Z | - |
dc.date.issued | 2011-12 | - |
dc.identifier.citation | Neurotoxicology December 2011 32(6):879-887 | en_US |
dc.identifier.issn | 0161-813X | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0161813X11000982 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/66051 | - |
dc.description.abstract | The neurotoxicity of L-3,4-dihydroxyphenylalanine (L-DOPA), one of the most important drugs for the treatment of Parkinson's disease, still remains controversial, although much more data on L-DOPA neurotoxicity have been presented. Considering the well known neuroprotective effects of erythropoietin (EPO), the inhibitory effects of EPO on L-DOPA neurotoxicity need to be evaluated. Neuronally differentiated PC12 (nPC12) cells were treated with different concentrations of L-DOPA and/or EPO for 24 h. Cell viability was evaluated using trypan blue, 4',6-diamidino-2-phenylindole (DAPI) and TUNEL staining, and cell counting. Free radicals and intracellular signaling protein levels were measured with 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) and Western blotting, respectively. L-DOPA reduced nPC12 cell viability at higher concentrations, but combined treatment with EPO and L-DOPA significantly restored cell viability. Free radicals and hydroxyl radical levels increased by L-DOPA were decreased after combined treatment of L-DOPA and EPO. Levels of survival-related intracellular signaling proteins decreased in nPC12 cells treated with 200 mu M L-DOPA but increased significantly in cells treated with 200 mu M L-DOPA and 5 mu M EPO. However, cleaved caspase-3, a death-related protein, increased in nPC12 cells treated with 200 mu M L-DOPA but decreased significantly in cells treated with 200 mu M L-DOPA and 5 mu M EPO. Pretreatment with LY294002, a phosphatidylinositol 3-kinase inhibitor, prior to combined treatment with EPO and L-DOPA almost completely blocked the protective effects of EPO. These results indicate that EPO can prevent L-DOPA neurotoxicity by activating the PI3K pathway as well as reducing oxidative stress. (C) 2011 Elsevier Inc. All rights reserved. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science B.V., Amsterdam. | en_US |
dc.subject | L-DOPA | en_US |
dc.subject | Erythropoietin | en_US |
dc.subject | Phosphatidylinositol 3-kinase | en_US |
dc.subject | Neurotoxicity | en_US |
dc.title | L-DOPA neurotoxicity is prevented by neuroprotective effects of erythropoietin | en_US |
dc.type | Article | en_US |
dc.relation.volume | 32 | - |
dc.identifier.doi | 10.1016/j.neuro.2011.05.009 | - |
dc.relation.page | 879-887 | - |
dc.relation.journal | NEUROTOXICOLOGY | - |
dc.contributor.googleauthor | Park, K. H. | - |
dc.contributor.googleauthor | Choi, N. Y. | - |
dc.contributor.googleauthor | Koh, S. H. | - |
dc.contributor.googleauthor | Park, H. H. | - |
dc.contributor.googleauthor | Kim, Y. S. | - |
dc.contributor.googleauthor | Kim, M. J. | - |
dc.contributor.googleauthor | Lee, S. J. | - |
dc.contributor.googleauthor | Yu, H. J. | - |
dc.contributor.googleauthor | Lee, K. Y. | - |
dc.contributor.googleauthor | Lee, Y. J. | - |
dc.relation.code | 2011207057 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | newdiaz | - |
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