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dc.contributor.author김철영-
dc.date.accessioned2018-04-12T06:55:54Z-
dc.date.available2018-04-12T06:55:54Z-
dc.date.issued2016-07-
dc.identifier.citationPLOS ONE, v. 11, No. 7, Article no. e0158796en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0158796-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/65638-
dc.description.abstractG protein-coupled receptor (GPR) 119 is expressed in pancreatic beta-cells and intestinal L cells, and is involved in glucose-stimulated insulin secretion and glucagon-like peptide-1 (GLP-1) release, respectively. Therefore, the development of GPR119 agonists is a potential treatment for type 2 diabetes. We screened 1500 natural plant extracts for GPR119 agonistic actions and investigated the most promising extract, that from Angelica dahurica (AD), for hypoglycemic actions in vitro and in vivo. Human GPR119 activation was measured in GeneBLAzer T-Rex GPR119-CRE-bla CHO-K1 cells; intracellular cAMP levels and insulin secretion were measured in INS-1 cells; and GLP-1 release was measured in GLUTag cells. Glucose tolerance tests and serum plasma insulin levels were measured in normal C57BL6 mice and diabetic db/db mice. AD extract-treated cells showed significant increases in GPR119 activation, intracellular cAMP levels, GLP-1 levels and glucose-stimulated insulin secretion as compared with controls. In normal mice, a single treatment with AD extract improved glucose tolerance and increased insulin secretion. Treatment with multiple doses of AD extract or n-hexane fraction improved glucose tolerance in diabetic db/db mice. Imperatorin, phellopterin and isoimperatorin were identified in the active fraction of AD extract. Among these, phellopterin activated GPR119 and increased active GLP-1 and insulin secretion in vitro and enhanced glucose tolerance in normal and db/db mice. We suggest that phellopterin might have a therapeutic potential for the treatment of type 2 diabetes.en_US
dc.description.sponsorshipThis research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI12C1564).en_US
dc.language.isoen_USen_US
dc.publisherPUBLIC LIBRARY SCIENCEen_US
dc.subjectPROTEIN-COUPLED RECEPTORen_US
dc.subjectGLYCEMIC CONTROLen_US
dc.subjectTYPE-2en_US
dc.subjectAGONISTSen_US
dc.subjectFURANOCOUMARINSen_US
dc.subjectPATHOGENESISen_US
dc.subjectRELEASEen_US
dc.subjectOBESITYen_US
dc.subjectAGENTSen_US
dc.subjectROOTSen_US
dc.titleAngelica dahurica Extracts Improve Glucose Tolerance through the Activation of GPR119en_US
dc.typeArticleen_US
dc.relation.no7-
dc.relation.volume11-
dc.identifier.doi10.1371/journal.pone.0158796-
dc.relation.page1-15-
dc.relation.journalPLOS ONE-
dc.contributor.googleauthorPark, EY-
dc.contributor.googleauthorKim, EH-
dc.contributor.googleauthorKim, CY-
dc.contributor.googleauthorKim, MH-
dc.contributor.googleauthorChoung, JS-
dc.contributor.googleauthorOh, YS-
dc.contributor.googleauthorMoon, HS-
dc.contributor.googleauthorJun, HS-
dc.relation.code2016007072-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidchulykim-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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