Optimal Formation of Genetically Modified and Functional Pancreatic Islet Spheroids by Using Hanging-Drop Strategy
- Title
- Optimal Formation of Genetically Modified and Functional Pancreatic Islet Spheroids by Using Hanging-Drop Strategy
- Author
- 이동윤
- Keywords
- GENE DELIVERY; ADENOASSOCIATED VIRUS; CELL COMMUNICATION; INSULIN-SECRETION; TRANSPLANTATION; DIFFERENTIATION; TRANSDUCTION; VECTOR
- Issue Date
- 2013-03
- Publisher
- Elsevier Science INC
- Citation
- Transplantation Proceedings, 2013, 45(2), P.605-610
- Abstract
- Background. Rejection and hypoxia are important factors causing islet loss at an early stage after pancreatic islet transplantation. Recently, islets have been dissociated into single cells for reaggregation into so-called islet spheroids. Herein, we used a hanging-drop strategy to form islet spheroids to achieve functional equivalence to intact islets.Methods. To obtain single islet cells, we dissociated islets with trypsin-EDTA digestion for 10 minutes. To obtain spheroids, we dropped various numbers of single cells (125, 250, or 500 cells/30 mu L drop) onto a Petri dish, that was inverted for incubation in humidified air containing 5% CO2 at 37 degrees C for 7 days. The aggregated spheroids in the droplets were harvested for further culture.Results. The size of the aggregated islet spheroids depended on the number of single cells (125-500 cells/30 mu L droplet). Their morphology was similar to that of intact islets without any cellular damage. When treated with various concentrations of glucose to evaluate responsiveness, their glucose-mediated stimulation index value was similar to that of intact islets, an observation that was attributed to strong cell-to-cell interactions in islet spheroids. However, islet spheroids aggregated in general culture dishes showed abnormal glucose responsiveness owing to weak cell-to-cell interactions. Cell-to-cell interactions in islet spheroids were confirmed with an anti-connexin-36 monoclonal antibody. Finally, nonviral poly(ethylene imine) mediated interleukin-10 cytokine gene delivered beforehand into dissociated single cells before formation of islet spheroids increased the gene transfection efficacy and interleukin-10 secretion from islet spheroids >4-fold compared with intact islets.Conclusion. These results demonstrated the potential application of genetically modified, functional islet spheroids with of controlled size and morphology using an hanging-drop technique.
- URI
- http://www.sciencedirect.com/science/article/pii/S0041134512013504?via%3Dihubhttp://hdl.handle.net/20.500.11754/55125
- ISSN
- 0041-1345
- DOI
- 10.1016/j.transproceed.2012.11.014
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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