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Biofilm-forming ability of Staphylococcus aureus strains isolated from human skin

Title
Biofilm-forming ability of Staphylococcus aureus strains isolated from human skin
Author
서경도
Keywords
Biofilm; Clinical isolates; Staphylococcus aureus; Crystal violet staining; Skin.
Issue Date
2013-08
Publisher
Elsevier Science B.V., Amsterdam.
Citation
Journal of Dermatological Science, August 2013, 71(2), p.130-137
Abstract
Background Staphylococcus aureus produces various toxins and enzymes, and its presence can exacerbate skin conditions. Previous studies have shown that S. aureus is involved in skin deterioration, even in normal tissue. Biofilm strains show much greater resistance to antimicrobial agents and therefore require a much higher concentration of biocide than planktonic counterparts.Objective As such, alternative strategies and more effective therapeutic agents against biofilm-producing S. aureus in skin are of great interest. Therefore, we turned our attention to differences in 50 clinical biofilm strains isolated from human facial skin.Methods Based on S. aureus density on facial skin, we divided donors into two groups: relatively low density (LSG) and high density (HSG). In general, strong biofilm-forming strains were detected in the HSG donors. Two strains from each of the groups were submitted to gene microarray analysis to investigate expression differences and confirmed by RT-PCR.Results In total, 111 of 7775 genes were differentially expressed between low (SA2 and SA7) vs. high (SA10 and SA33) biofilm-forming clinical strains. These genes include already well-known as biofilm formation related genes like icaABCD and lrgAB, and newly identified genes (sdrC, sspBCP) by RT-PCR. Comparison of gene expression differences between the two groups available at NCBI Gene Expression Omnibus accession number GSE44268.Conclusion Our results suggest that S. aureus density in the skin is closely related to biofilm-forming ability, and we have identified several potential target genes that may be involved in regulating biofilm formation in situ.
URI
https://www.sciencedirect.com/science/article/pii/S0923181113001199http://hdl.handle.net/20.500.11754/54802
ISSN
0923-1811
DOI
10.1016/j.jdermsci.2013.04.004
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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