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The novel vaccine peptide GV1001 effectively blocks beta-amyloid toxicity by mimicking the extra-telomeric functions of human telomerase reverse transcriptase

Title
The novel vaccine peptide GV1001 effectively blocks beta-amyloid toxicity by mimicking the extra-telomeric functions of human telomerase reverse transcriptase
Author
고성호
Keywords
Peptide; Vaccine; GV1001; beta-Amyloid; Oligomer; Neural stem cells
Issue Date
2014-06
Publisher
ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
Citation
NEUROBIOLOGY OF AGING, 35(6), p.1255-1274
Abstract
GV1001 is a 16-amino-acid vaccine peptide derived from the human telomerase reverse transcriptase sequence. We investigated the effects of GV1001 against beta-amyloid (A beta) oligomer-induced neurotoxicity in rat neural stem cells (NSCs). Primary culture NSCs were treated with several concentrations of GV1001 and/or A beta(25-35) oligomer for 48 hours. GV1001 protected NSCs against the A beta(25-35) oligomer in a concentration-dependent manner. A beta(25-35) concentration dependently decreased viability, proliferation, and mobilization of NSCs and GV1001 treatment restored the cells to wild-type levels. A beta(25-35) increased free radical levels in rat NSCs while combined treatment with GV1001 significantly reduced these levels. In addition, GV1001 treatment of A beta(25-35) injured NSCs increased the expression level of survival-related proteins, including mitochondria-associated survival proteins, and decreased the levels of death and inflammation-related proteins, including mitochondria-associated death proteins. Together, these results suggest that GV1001 possesses neuroprotective effects against A beta(25-35) oligomer in NSCs and that these effects are mediated through mimicking the extra-telomeric functions of human telomerase reverse transcriptase, including the induction of cellular proliferation, anti-apoptotic effects, mitochondrial stabilization, and anti-aging and anti-oxidant effects. (C) 2014 Elsevier Inc. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S019745801300657X?via%3Dihubhttp://hdl.handle.net/20.500.11754/54698
ISSN
0197-4580; 1558-1497
DOI
10.1016/j.neurobiolaging.2013.12.015
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > ETC
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