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Transplanted human amniotic epithelial cells secrete paracrine pro-angiogenic cytokines in rat model of myocardial infarction

Title
Transplanted human amniotic epithelial cells secrete paracrine pro-angiogenic cytokines in rat model of myocardial infarction
Author
송이선
Keywords
Amniotic epithelial cells; Myocardial infarction (MI); Paracrine; Cytokine
Issue Date
2014-11
Publisher
Medline
Citation
Cell Transplant. 2015, 24(10), P.2055-2119
Abstract
Human amniotic epithelial cells (h-AECs) have been shown to differentiate into cardiomyocyte-like cells in vivo that can regenerate myocardial tissue and improve cardiac function in a rat model of myocardial infarction (MI). In this study, we investigated the paracrine factors released from h-AECs under hypoxic conditions to elucidate the possible mechanisms underlying this previously reported phenomenon of h-AEC-mediated cardiac repair. We used hypoxic cell culture conditions to simulate myocardial infarction in vitro. In comparison to normal conditions, we found that h-AECs secreted higher levels of several cytokines, including angiogenin (ANG), epidermal growth factor (EGF), interleukin (IL)-6, and monocyte chemoattractant protein (MCP)-1. To determine whether transplanted h-AECs express these proangiogenic cytokines in vivo, we ligated the coronary artery of rats to cause MI and injected either h-AECs or saline into the infarcted area. We found that the infarct and border zones of rat myocardium treated with h-AECs had higher expression levels of the human-origin cytokines ANG, EGF, IL-6, and MCP-1 compared to the tissues of saline-treated rats. In conclusion, h-AECs secreted proangiogenic cytokines in a rat model of MI, which may suggest that the paracrine effect by h-AECs could regenerate myocardial tissue and improve cardiac function.
URI
http://journals.sagepub.com/doi/10.3727/096368914X685609http://hdl.handle.net/20.500.11754/54341
ISSN
0963-6897
DOI
10.3727/096368914X685609
Appears in Collections:
RESEARCH INSTITUTE[S](부설연구소) > ETC
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