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Neuroprotective effects of JGK-263 in transgenic SOD1-G93A mice of amyotrophic lateral sclerosis

Title
Neuroprotective effects of JGK-263 in transgenic SOD1-G93A mice of amyotrophic lateral sclerosis
Author
김승현
Keywords
JGK-263; Glycogen synthase kinase-3; GSK-3 beta inhibitor; Amyotrophic lateral sclerosis; ALS; Transgenic mouse
Issue Date
2014-05
Publisher
ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Citation
JOURNAL OF THE NEUROLOGICAL SCIENCES, 2014, 340(1-2), P.112-116
Abstract
Background: Glycogen synthase kinase-3 beta (GSK-3 beta) activity plays a central role in motor neuron degeneration. GSK-3 beta inhibitors have been shown to prolong motor neuron survival and suppress disease progression in amyotrophic lateral sclerosis (ALS). In this study, we evaluated the therapeutic effects of a new GSK-3b inhibitor, JGK-263, on ALS in G93A SOD1 transgenic mice. Methods: Previously, biochemical efficacy of JGK-263 was observed in normal and mutant (G93A) hSOD1-transfected motor neuronal cell lines (NSC34). Based on these previous results, we administered JGK-263 orally to 93 transgenic mice with the human G93A-mutated SOD1 gene. The mice were divided into three groups: a group administered 20 mg/kg JGK-263, a group administered 50 mg/kg JGK-263, and a control group not administered with JGK-263. Clinical status, rotarod test, and survival rates of transgenic mice with ALS were evaluated. Sixteen mice from each group were selected for further biochemical study that involved examination of motor neuron count, apoptosis, and cell survival signals. Results: JGK-263 administration remarkably improved motor function and prolonged the time until symptom onset, rotarod failure, and death in transgenic mice with ALS compared to control mice. In JGK-263 groups, choline acetyltransferase (ChAT) staining in the ventral horn of the lower lumbar spinal cord showed a large number of motor neurons, suggesting normal morphology. The neuroprotective effects of JGK-263 in ALS mice were also suggested by western blot analysis of spinal cord tissues in transgenic mice. Conclusion: These results suggest that JGK-263, an oral GSK-3 beta inhibitor, is promising as a novel therapeutic agent for ALS. Still, further biochemical studies on the underlying mechanisms and safety of JGK-263 are necessary. (C) 2014 Elsevier B.V. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0022510X14001518http://hdl.handle.net/20.500.11754/54229
ISSN
0022-510X; 1878-5883
DOI
10.1016/j.jns.2014.03.008
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > ETC
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