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Wafer-scale nanowell array patterning based electrochemical mpedimetric immunosensor

Title
Wafer-scale nanowell array patterning based electrochemical mpedimetric immunosensor
Author
박진구
Keywords
Nanowell arrays (NWA) immunosensor; Wafer-scale nanopatterning; Electrochemical impedance spectroscopy (EIS); Stress-induced-phosphoprotein-1 (STIP-1)
Issue Date
2013-09
Publisher
Elsevier Science B.V
Citation
Journal of Biotechnology, 2013, 168(4), P.584-588
Abstract
We have reported that nanowell array (NWA) can enhance electrochemical detection of molecular binding events by controlling the binding sites of the captured molecules. Using NWA biosensor based amperometric analysis, we have detected biological macromolecules such as DNA, protein or aptamers at low concentrations. In this research, we developed an impedimetric immunosensor based on wafer-scale NWA for electrochemical detection of stress-induced-phosphoprotein-1 (STIP-1). In order to develop NWA sensor through the cost-effective combination of high-throughput nanopattern, the NWA electrode was fabricated on Si wafer by krypton-fluoride (KrF) stepper semiconductor process. Finally, 12,500,000 ea nanowell with a 500 nm diameter was fabricated on 4 mm × 2 mm substrate. Next, by using these electrodes, we measured impedance to quantify antigen binding to the immunoaffinity layer. The limit of detection (LOD) of the NWA was improved about 100-fold compared to milli-sized electrodes (4 mm × 2 mm) without an NWA. These results suggest that wafer-scale NWA immunosensor will be useful for biosensing applications because their interface response is appropriate for detecting molecular binding events.
URI
https://www.sciencedirect.com/science/article/pii/S0168165613003805http://hdl.handle.net/20.500.11754/53934
ISSN
0168-1656
DOI
10.1016/j.jbiotec.2013.08.029
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > ETC
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