Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 류재숙 | - |
dc.date.accessioned | 2018-03-29T05:04:35Z | - |
dc.date.available | 2018-03-29T05:04:35Z | - |
dc.date.issued | 2016-05 | - |
dc.identifier.citation | PROSTATE, v. 76, NO 10, Page. 885-896 | en_US |
dc.identifier.issn | 0270-4137 | - |
dc.identifier.issn | 1097-0045 | - |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/abs/10.1002/pros.23178 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/53656 | - |
dc.description.abstract | BACKGROUND. Trichomonas vaginalis is a sexually transmitted protozoan parasite that causes vaginitis in women, and urethritis and prostatitis in men. IL-1 beta is synthesized as immature pro-IL-1 beta, which is cleaved by activated caspase-1. Caspase-1 is, in turn, activated by a multi-protein complex known as an inflammasome. In this study, we investigated the inflammatory response of a prostate epithelial cell line (RWPE-1) to T. vaginalis and, specifically, the capacity of T. vaginalis to activate the NLRP3 inflammasome. METHODS. RWPE-1 cells were stimulated by live T. vaginalis, and subsequent expression of pro-IL-1 beta, IL-1 beta, NLRP3, ASC and caspase-1 was determined by real-time PCR and Western blotting. IL-1 beta and caspase-1 production was also measured by ELISA. To evaluate the effects of NLRP3 and caspase-1 on IL-1 beta production, the activated RWPE-1 cells were transfected with small interfering RNAs to silence the NLRP3 and caspase-1 genes. Activation of the NLRP3 inflammasome was observed by fluorescence microscopy. Intracellular reactive oxygen species (ROS) were evaluated by spectrofluorometry. RESULTS. When RWPE-1 cells were stimulated with live T. vaginalis, the mRNA and protein expression of IL-1 beta, NLRP3, ASC, and caspase-1 increased. Moreover, silencing of NLRP3 and caspase-1 attenuated T. vaginalis-induced IL-1 beta secretion. The NADPH oxidase inhibitor DPI and high extracellular potassium ion suppressed the production of IL-1 beta, caspase-1, and the expression of NLRP3 and ASC proteins. The specific NF-kappa B inhibitor, Bay 11-7082, inhibited IL-1 beta production, and also inhibited the production of caspase-1, ASC and NLRP3 proteins. CONCLUSIONS. T. vaginalis induces the formation of the NLRP3 inflammasome in human prostate epithelial cells via ROS and potassium ion efflux, and this results in IL-1 beta production. This is the first evidence for activation of the NLRP3 inflammasome in the inflammatory response by prostate epithelial cells infected with T. vaginalis. (C) 2016 Wiley Periodicals, Inc. | en_US |
dc.description.sponsorship | Grant sponsor: National Research Foundation of Korea (NRF) funded by the Korean Government (MSIP); Grant number: NRF-2014R1A2A2A01005449. | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-BLACKWELL | en_US |
dc.subject | prostate epithelial cell | en_US |
dc.subject | Trichomonas vaginalis | en_US |
dc.subject | IL-1 beta | en_US |
dc.subject | NLRP3 inflammasome | en_US |
dc.title | Trichomonas vaginalis Induces IL-1 beta Production in a Human Prostate Epithelial Cell Line by Activating the NLRP3 Inflammasome Via Reactive Oxygen Species and Potassium Ion Efflux | en_US |
dc.type | Article | en_US |
dc.relation.no | 10 | - |
dc.relation.volume | 76 | - |
dc.identifier.doi | 10.1002/pros.23178 | - |
dc.relation.page | 885-896 | - |
dc.relation.journal | PROSTATE | - |
dc.contributor.googleauthor | Gu, Na-Yeong | - |
dc.contributor.googleauthor | Kim, Jung-Hyun | - |
dc.contributor.googleauthor | Han, Ik-Hwan | - |
dc.contributor.googleauthor | Im, Su-Jeong | - |
dc.contributor.googleauthor | Seo, Min-Young | - |
dc.contributor.googleauthor | Chung, Yong-Hoon | - |
dc.contributor.googleauthor | Ryu, Jae-Sook | - |
dc.relation.code | 2016002486 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jsryu | - |
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