Trichomonas vaginalis Induces IL-1 beta Production in a Human Prostate Epithelial Cell Line by Activating the NLRP3 Inflammasome Via Reactive Oxygen Species and Potassium Ion Efflux

Title
Trichomonas vaginalis Induces IL-1 beta Production in a Human Prostate Epithelial Cell Line by Activating the NLRP3 Inflammasome Via Reactive Oxygen Species and Potassium Ion Efflux
Author
류재숙
Keywords
prostate epithelial cell; Trichomonas vaginalis; IL-1 beta; NLRP3 inflammasome
Issue Date
2016-05
Publisher
WILEY-BLACKWELL
Citation
PROSTATE, v. 76, NO 10, Page. 885-896
Abstract
BACKGROUND. Trichomonas vaginalis is a sexually transmitted protozoan parasite that causes vaginitis in women, and urethritis and prostatitis in men. IL-1 beta is synthesized as immature pro-IL-1 beta, which is cleaved by activated caspase-1. Caspase-1 is, in turn, activated by a multi-protein complex known as an inflammasome. In this study, we investigated the inflammatory response of a prostate epithelial cell line (RWPE-1) to T. vaginalis and, specifically, the capacity of T. vaginalis to activate the NLRP3 inflammasome. METHODS. RWPE-1 cells were stimulated by live T. vaginalis, and subsequent expression of pro-IL-1 beta, IL-1 beta, NLRP3, ASC and caspase-1 was determined by real-time PCR and Western blotting. IL-1 beta and caspase-1 production was also measured by ELISA. To evaluate the effects of NLRP3 and caspase-1 on IL-1 beta production, the activated RWPE-1 cells were transfected with small interfering RNAs to silence the NLRP3 and caspase-1 genes. Activation of the NLRP3 inflammasome was observed by fluorescence microscopy. Intracellular reactive oxygen species (ROS) were evaluated by spectrofluorometry. RESULTS. When RWPE-1 cells were stimulated with live T. vaginalis, the mRNA and protein expression of IL-1 beta, NLRP3, ASC, and caspase-1 increased. Moreover, silencing of NLRP3 and caspase-1 attenuated T. vaginalis-induced IL-1 beta secretion. The NADPH oxidase inhibitor DPI and high extracellular potassium ion suppressed the production of IL-1 beta, caspase-1, and the expression of NLRP3 and ASC proteins. The specific NF-kappa B inhibitor, Bay 11-7082, inhibited IL-1 beta production, and also inhibited the production of caspase-1, ASC and NLRP3 proteins. CONCLUSIONS. T. vaginalis induces the formation of the NLRP3 inflammasome in human prostate epithelial cells via ROS and potassium ion efflux, and this results in IL-1 beta production. This is the first evidence for activation of the NLRP3 inflammasome in the inflammatory response by prostate epithelial cells infected with T. vaginalis. (C) 2016 Wiley Periodicals, Inc.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/pros.23178http://hdl.handle.net/20.500.11754/53656
ISSN
0270-4137; 1097-0045
DOI
10.1002/pros.23178
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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