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A comparison of oligonucleotide-based microarray and real-time PCR for the detection of sexually transmitted infections

Title
A comparison of oligonucleotide-based microarray and real-time PCR for the detection of sexually transmitted infections
Author
황승용
Keywords
STIs; Real-time PCR; Microarray; DNA chip; Molecular diagnostics
Issue Date
2013-03
Publisher
Korean Biochip Society
Citation
Biochip journal, 2013, 7(1), P.68-74
Abstract
Many diagnostic methods for sexually transmitted infections (STIs) have been developed. Because various infection agents are associated with STIs, and because infected persons sometimes show no symptoms, the diagnosis of STIs using nucleic acid amplification tests(NAATs) has required not only simultaneous multi-targeting, but also sensitive detection. Here, we compare microarray and real-time PCR for the detection of three common STIs agents, Ureaplasma urealyticum, Mycoplasma genitalium, and Chlamydia trachomatis, using human urine samples. The detection results showed that microarray and real-time PCR technology are both effective tools for the detection of STI agents. In conclusion, real-time PCR detection offers more sensitivity and specificity than microarray, because of the quantitative method employed. But, microarray offers better performance, in terms of high-throughput and simultaneous multi-targeting.
URI
http://link.springer.com/article/10.1007%2Fs13206-013-7111-1http://hdl.handle.net/20.500.11754/53168
ISSN
1976-0280
DOI
10.1007/s13206-013-7111-1
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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