Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 배재만 | - |
dc.date.accessioned | 2018-03-27T00:56:52Z | - |
dc.date.available | 2018-03-27T00:56:52Z | - |
dc.date.issued | 2013-04 | - |
dc.identifier.citation | Journal of Clinical Virology , 2013, 57(2), P.130-135 | en_US |
dc.identifier.issn | 1386-6532 | - |
dc.identifier.uri | http://www.journalofclinicalvirology.com/article/S1386-6532(13)00038-3/fulltext | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/52834 | - |
dc.description.abstract | Background: The need for accurate genotyping of human papillomavirus (HPV) infections is becoming increasingly important as HPV is the primary cause of cervical cancer worldwide. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry-based restriction fragment mass polymorphism (RFMP) assay provides accurate, broad-spectrum, high-throughput genotyping of HPV.Objectives: We evaluated the clinical performance of the RFMP assay compared to a commercially available Roche linear array HPV genotyping test (LA) for detecting and genotyping of HPV.Study design: The RFMP assay and the LA were compared for detecting and genotyping HPV among a cohort of 244 liquid-based cytology samples.Results: Overall, 216 specimens (93.1%, kappa = 0.86) generated concordant results for the presence or absence of high-risk HPV (HR-HPV) by the two assays. The RFMP assay and the LA assay generated concordant, compatible, and discordant genotyping results for 79.3, 9.9, and 10.8%, respectively. The diagnostic sensitivity and specificity of RFMP and LA for the cervical lesions of squamous cell carcinoma (SCC) were similar, at 92.9 and 85.0% (RFMP) and 92.9 and 83.8% (LA), respectively. In addition, the odds ratio for SCC with HR-HPV positivity estimated by the RFMP assay (73.7, 95% CI: 8.9-3173.3) was higher than the LA assay (67.0, 95% CI: 8.2-2887.0).Conclusions: The RFMP and the LA assays were highly comparable with regard to detection and genotyping analysis of HPV. The sensitivity and specificity of RFMP assay for the detection of HR-HPV in various levels of cervical lesions seems to be valuable in the monitoring of HPV-associated cervical cancer. (c) 2013 Elsevier B.V. All rights reserved. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science B.V | en_US |
dc.subject | Clinical | en_US |
dc.subject | Genotype | en_US |
dc.subject | Human papillomavirus | en_US |
dc.subject | Mass spectrometry | en_US |
dc.title | Comparison of the clinical performance of restriction fragment mass polymorphism (RFMP) and Roche linear array HPV test assays for HPV detection and genotyping | en_US |
dc.type | Article | en_US |
dc.relation.volume | 57 | - |
dc.identifier.doi | 10.1016/j.jcv.2013.01.014 | - |
dc.relation.page | 130-135 | - |
dc.relation.journal | JOURNAL OF CLINICAL VIROLOGY | - |
dc.contributor.googleauthor | Lee, Hyo-Pyo | - |
dc.contributor.googleauthor | Cho, Woojae | - |
dc.contributor.googleauthor | Bae, Jae-Man | - |
dc.contributor.googleauthor | Shin, Ji Young | - |
dc.contributor.googleauthor | Shin, Soo-Kyung | - |
dc.contributor.googleauthor | Hwang, Sun Young | - |
dc.contributor.googleauthor | Min, Kyung Tae | - |
dc.contributor.googleauthor | Kim, Soo Nyung | - |
dc.contributor.googleauthor | Lee, Sun Joo | - |
dc.contributor.googleauthor | Kim, Soo-Ok | - |
dc.contributor.googleauthor | Yoo, Wang Don | - |
dc.contributor.googleauthor | Hong, Sun Pyo | - |
dc.relation.code | 2013010581 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | obgybae | - |
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