Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 채영규 | - |
dc.date.accessioned | 2018-03-26T05:56:04Z | - |
dc.date.available | 2018-03-26T05:56:04Z | - |
dc.date.issued | 2014-10 | - |
dc.identifier.citation | Current microbiology ,Vol.69,No.4 [2014] ,pp.436-444 | en_US |
dc.identifier.issn | 0343-8651 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007/s00284-014-0606-8 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/52405 | - |
dc.description.abstract | RelA and SpoT synthesize ppGpp, a key effector molecule that facilitates the adaptation of bacteria to nutrient starvation and other stresses, known as the stringent response. To investigate the role of Rsh Bant , a putative RelA/SpoT homolog (encoded by BAS4302) in Bacillus anthracis, we examined the alteration of the secretome profiles after the overexpression of a functional His-Rsh Bant protein in the B. anthracis strain Sterne at the stationary growth phase. In the ppGpp-deficient E. coli mutant strain CF1693, overexpression of Rsh Bant restored a ppGpp-dependent growth defect on minimal glucose media. The secretome profiles obtained using a two-dimensional electrophoresis (2-DE) analysis were altered by overexpression of Rsh Bant in B. anthracis. Among the 66 protein spots differentially expressed >1.5-fold, the 29 proteins were abundant for further identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Functional categorization of those proteins implicated their involvement in various biological activities. Taken together, our results imply that overexpression of a functional His-Rsh Bant can lead to the increased levels of intracellular ppGpp in B. anthracis, resulting in the significant changes in its secretome profiling. The stringent response-controlled proteins identified are likely useful as potential targets for serodiagnostic applications. | en_US |
dc.description.sponsorship | This work was funded through a grant from the National Research Foundation of Korea (NRF) through the Korean government (MSIP) (No. 2011-0030049). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer Science + Business Media | en_US |
dc.subject | Protein Spot | en_US |
dc.subject | Stationary Growth Phase | en_US |
dc.subject | Bacillus Anthracis | en_US |
dc.subject | Stringent Response | en_US |
dc.subject | Brucella Abortus | en_US |
dc.title | Identification of Stringent Response-Related and Potential Serological Proteins Released from Bacillus anthracis Overexpressing the RelA/SpoT Homolog, Rsh(Bant) | en_US |
dc.title.alternative | SpoT Homolog, Rsh(Bant) | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 69 | - |
dc.identifier.doi | 10.1007/s00284-014-0606-8 | - |
dc.relation.page | 436-444 | - |
dc.relation.journal | CURRENT MICROBIOLOGY | - |
dc.contributor.googleauthor | Kim, Se-Kye | - |
dc.contributor.googleauthor | Park, Moon-Kyoo | - |
dc.contributor.googleauthor | Kim, Sang-Hoon | - |
dc.contributor.googleauthor | Oh, Kwang-Gun | - |
dc.contributor.googleauthor | Jung, Kyoung-Hwa | - |
dc.contributor.googleauthor | Hong, Chong-Hae | - |
dc.contributor.googleauthor | Yoon, Jang W. | - |
dc.contributor.googleauthor | Chai, Young-Gyu | - |
dc.relation.code | 2014028091 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | ygchai | - |
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