Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최호순 | - |
dc.date.accessioned | 2018-03-26T02:39:32Z | - |
dc.date.available | 2018-03-26T02:39:32Z | - |
dc.date.issued | 2013-03 | - |
dc.identifier.citation | Liver International, 2013, 33(4), P.535-543 | en_US |
dc.identifier.issn | 1478-3223 | - |
dc.identifier.issn | 1478-3231 | - |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/abs/10.1111/liv.12110 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/52181 | - |
dc.description.abstract | Background 5-hydroxytryptamine (5- HT) receptors are upregulated in activated hepatic stellate cells ( HSCs), and are therefore thought to play an important role in their activation. Aim The aim of this study was to determine whether 5- HT2A receptor antagonists affect the activation or apoptosis of HSCs in vitro and/or in vivo. Methods For the in vitro experiments, the viability, apoptosis and wound healing ability of LX-2 cells were examined after treatment with various 5- HT2A receptor antagonists. Levels of HSC activation markers (procollagen type I, α- SMA, TGF-β and Smad 2/3) were measured. For in vivo experiments, rats were divided into three groups: (i) a control group, (ii) a disease group, in which cirrhosis was induced by thioacetamide (iii) a treatment group, in which cirrhosis was induced and a 5- HT2A receptor antagonist (sarpogrelate, 30 mg/kg) was administered. Results 5-HT2A, but not 5-HT2B receptor mRNA increased with time upon HSC activation. 5-HT2A receptor antagonists (ketanserin and sarpogrelate) inhibited viability and wound healing in LX-2 cells and induced apoptosis. Expression of α-SMA and procollagen type I was also inhibited. In the in vivo study, lobular inflammation was reduced in the sarpogrelate-treated group, but there was only slight and statistically insignificant attenuation of periportal fibrosis. Expression of α-SMA, TGF-β and Smad 2/3 was also reduced in the treatment group. Conclusions 5- HT2A receptor antagonists can reduce inflammation and the activation of HSCs in this cirrhotic model. | en_US |
dc.description.sponsorship | This work was supported by the National ResearchFoundation of Korea 2011-0007127. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley-Blackwell | en_US |
dc.subject | hepatic stellate cell | en_US |
dc.subject | fibrosis | en_US |
dc.subject | sarpogrelate | en_US |
dc.subject | 5-HT | en_US |
dc.subject | 5-HT2A receptor | en_US |
dc.title | 5-HT2A receptor antagonists inhibit hepatic stellate cell activation and facilitate apoptosis | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 33 | - |
dc.identifier.doi | 10.1111/liv.12110 | - |
dc.relation.page | 535-543 | - |
dc.relation.journal | LIVER INTERNATIONAL | - |
dc.contributor.googleauthor | Kim, DongChan | - |
dc.contributor.googleauthor | Jun, DaeWon | - |
dc.contributor.googleauthor | Kwon, YoungIl | - |
dc.contributor.googleauthor | Lee, KangNyeong | - |
dc.contributor.googleauthor | Lee, HangLak | - |
dc.contributor.googleauthor | Lee, OhYoung | - |
dc.contributor.googleauthor | Yoon, ByungChul | - |
dc.contributor.googleauthor | Choi, HoSoon | - |
dc.contributor.googleauthor | Kim, EunKyung | - |
dc.relation.code | 2013011164 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | hschoi96 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.