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Effects of Size, Impurities, and Citrate Capping on the Toxicity of Manufactured Silver Nano-particles to Larval Zebrafish (Danio rerio)

Title
Effects of Size, Impurities, and Citrate Capping on the Toxicity of Manufactured Silver Nano-particles to Larval Zebrafish (Danio rerio)
Author
윤태현
Keywords
Danio rerio; gill; nano hazard; particle size; silver nanoparticles
Issue Date
2013-09
Publisher
한국환경보건학회(Korean Society of Environmental Health)
Citation
한국환경보건학회지(Journal of environmental health sciences), 2013, 39(4), P.369-375
Abstract
Objectives: This study was conducted to identify factors determining the toxicity of manufactured silver nanoparticles(AgNPs) on aquatic organisms.Methods: For this purpose, we prepared several AgNPs with varied characteristics, including hydrodynamic size(nano-ABCAgCit vs -sized-ABCAgCit), impurities (ABCAg stock vs ABCAg), and citrate capping (ABCAgCit), using a commercially available manufactured AgNP (ABCAg stock). Acute tests were conducted using larval zebrafish (DaniorerioI). In addition, in order to determine the ecotoxicological potentials of various capping agents, toxicity testswere conducted with microbes, waterfleas, and fish for eight different capping agents that are used for NPs.Results: The toxicity of AgNPs in terms of 96 h fish LC50 increased in the following order: ABCAg stock < ABCAg= ABCAgCit = nano-ABCAgCit < μ-sized-ABCAgCit < AgNO3. After removing impurities by dialysis, 96 h LC50 valuedecreased significantly from 126.6 μg/L (95% confidence intervals [CI]: 107.0-146.2) (ABCAg stock) to 78.6 μg/L(CI: 72.7-84.8) (ABCAg). For μ-sized-ABCAgCit (ranging between 3.9 and 40.6 nm) and ABCAgCit (40.6 nm and9.1 μm), the 96 h LC50 of the former (43.9 μg/L, CI: 36.0-51.7) was approximately two-fold lower than that ofthe latter (87.0 μg/L, CI: 73.5-100.3).Conclusions: In this study, we found that for acute lethality, the contribution of impurities and particle size wassignificant, but that of citrate was negligible.
URI
http://koreascience.or.kr/article/ArticleFullRecord.jsp?cn=HROSBE_2013_v39n4_369
ISSN
1738-4087
DOI
10.5668/JEHS.2013.39.4.369
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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