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New ganglio-tripod amphiphiles (TPAs) for membrane protein solubilization and stabilization: implications for detergent structure-property relationships

Title
New ganglio-tripod amphiphiles (TPAs) for membrane protein solubilization and stabilization: implications for detergent structure-property relationships
Author
김진웅
Keywords
MUSCARINIC ACETYLCHOLINE-RECEPTOR
Issue Date
2014-11
Publisher
ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Citation
ORGANIC & BIOMOLECULAR CHEMISTRY, 권: 12, 호: 42, 페이지: 8480-8487
Abstract
Detergents are widely used for membrane protein research; however, membrane proteins encapsulated in micelles formed by conventional detergents tend to undergo structural degradation, necessitating the development of new agents with enhanced efficacy. Here we prepared several hydrophobic variants of ganglio-tripod amphiphiles (TPAs) derived from previously reported TPAs and evaluated for a multi-subunit, pigment protein superassembly. In this study. TPA-16 was found to be most efficient in protein solubilization while TPA-15 proved most favourable in long-term protein stability. The current study combined with previous TPA studies enabled us to elaborate on a few detergent structure-property relationships that could provide useful guidelines for novel amphiphile design.
URI
http://pubs.rsc.org/en/Content/ArticleLanding/2014/OB/C4OB01375A#!divAbstracthttp://hdl.handle.net/20.500.11754/51266
ISSN
1477-0520; 1477-0539
DOI
10.1039/c4ob01375a
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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