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dc.contributor.author윤채옥-
dc.date.accessioned2018-03-23T04:41:44Z-
dc.date.available2018-03-23T04:41:44Z-
dc.date.issued2014-04-
dc.identifier.citationLIVER INTERNATIONAL; APR 2014, 34, 4, p632-p642en_US
dc.identifier.issn1478-3223-
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/abs/10.1111/liv.12375-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/51198-
dc.description.abstractBackground & Aims Hypoxia-inducible factor-1 alpha (HIF-1 alpha), a key transcription factor in the cellular response to hypoxia, and interleukin 8 (IL-8), a key mediator of angiogenesis, are important in cancerous tumour growth. In this study, we evaluated the effects of HIF-1 alpha and IL-8 knockdown on angiogenesis and tumour growth in hepatocellular carcinoma (HCC). Methods Hepatocellular carcinoma cell lines were infected with adenoviruses expressing small-hairpin RNA (shRNA) specific for HIF-1 alpha or IL-8, cultured under hypoxic conditions (1% O2), and examined for their levels of HIF-1 alpha, IL-8, and angiogenesis factors using immunoblot. The effects of adenovirus-mediated shRNA-induced HIF-1 alpha and IL-8 knockdown on tumour growth and angiogenesis were also investigated in a subcutaneous Hep3B-tumour mouse model. Results Hypoxia-inducible factor-1 alpha knockdown directly repressed tumour growth, whereas IL-8 knockdown indirectly repressed tumour growth. Combined knockdown of HIF-1 alpha and IL-8 increased survival rates of mice. HIF-1 alpha and IL-8 knockdown also decreased microvessel density and tumour volume in vivo. Similarly, HIF-1 alpha and IL-8 knockdown inhibited the angiogenic effects of HCC cell-conditioned media on tube formation and invasion by endothelial cells in vitro. Conclusion These findings indicate that shRNA-induced HIF-1 alpha and IL-8 knockdown inhibit angiogenesis and tumour growth in HCC. Further development of HIF-1 alpha and IL-8 shRNA technologies could lead to effective therapies for HCC.en_US
dc.description.sponsorshipThis research was supported byBasic Science Research Program through the NationalResearch Foundation of Korea NRF funded by the Min-istry of Educatio n, Science and Technology (NRF-2010-0011791, 2011-0005051)en_US
dc.language.isoenen_US
dc.publisherWILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USAen_US
dc.subjectangiogenesisen_US
dc.subjectinterleukin-8en_US
dc.subjecthepatocellular carcinomaen_US
dc.subjecthypoxia inducible factor-1 alphaen_US
dc.subjecttumour xenografen_US
dc.titleInhibition of tumour angiogenesis and growth by small hairpin HIF-1 alpha and IL-8 in hepatocellular carcinomaen_US
dc.typeArticleen_US
dc.relation.no4-
dc.relation.volume34-
dc.identifier.doi10.1111/liv.12375-
dc.relation.page632-642-
dc.relation.journalLIVER INTERNATIONAL-
dc.contributor.googleauthorKim, Kyung Sik-
dc.contributor.googleauthorChoi, Sung Hoon-
dc.contributor.googleauthorHan, Kwang-Hyub-
dc.contributor.googleauthorPark, Jun Yong-
dc.contributor.googleauthorKim, Do Young-
dc.contributor.googleauthorAhn, Sang Hoon-
dc.contributor.googleauthorKim, Seung Up-
dc.contributor.googleauthorRo, Simon Weonsang-
dc.contributor.googleauthorKim, Seungtaek-
dc.contributor.googleauthorYun, Chae-Ok-
dc.relation.code2014035497-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidchaeok-
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COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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