Identification of ultraviolet B radiation-induced microRNAs in normal human dermal papilla cells

Abstract

Background: Ultraviolet (UV) radiation is associated with many processes involved in aging such as DNA damage, regulation of cell growth and apoptosis. microRNAs(miRNAs) have been shown to function as primary regulators of a variety of biological processes, including proliferation, differentiation, and apoptosis. However, the biological significance of miRNAs in aging process by UV in normal human dermal papilla cells(nHDPs) remains unclear. Objectives: We sought to evaluate the changes of miRNAs to UV radiation in nHDPs. Methods: Normal human dermal papillas (nHDPs) were irradiated with various doses of UV. With miRNA microarray, the changes of miRNAs were evaluated. In addition, bioinformatics tools were applied to predict the target genes of these differentially expressed miRNAs. Results: In this study, UVB exposure induced growth arrest and cell death in nHDPs in a dose-dependent manner. Moreover, with miRNA microarray analysis, 42 miRNAs either up- or down-regulated were identified. And the associated biological functions of the target genes were categorized by gene ontology analysis. Conclusion: UVB-induced cell growth arrest and cell death may be mediated by miRNAs involved in cellular stimulation and communication in HDPs.