43 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author이민형-
dc.date.accessioned2018-03-23T01:11:23Z-
dc.date.available2018-03-23T01:11:23Z-
dc.date.issued2013-11-
dc.identifier.citationJournal of Drug Targeting, 2013, 21(9), P.830-836en_US
dc.identifier.issn1061-186X-
dc.identifier.issn1029-2330-
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.3109/1061186X.2013.829073-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/50964-
dc.description.abstractSolid tumors have hypoxic regions in their cores, due to low blood supply levels. Therefore, hypoxia-specific gene regulation systems have been developed for tumor-specific gene therapy. In this study, the oxygen-dependent degradation (ODD) domain on activating transcription factor-4 (ATF4) was evaluated for post-translational regulation of proteins. The ATF4 ODD cDNA was amplified by RT-PCR, and a luciferase plasmid containing the ATF4 ODD domain, pSV-Luc-ATF4-ODD, was constructed. Luciferase expression was induced under hypoxia by the ATF4 ODD domain in transfection assays into N2A neuroblastoma cells, C6 glioblastoma cells, and U87 glioblastoma cells. In the transfection assay with pSV-Luc-ATF4-ODD, RT-PCR results showed that the mRNA level did not change under hypoxia. This suggests that the induction of luciferase under hypoxia was mediated by post-translational regulation. A plasmid expressing thymidine kinase from herpes simplex virus (HSV-tk), pSV-HSVtk-ATF4-ODD, was constructed with the ATF4 ODD cDNA. The transfection assay with pSV-TK-ATF4-ODD showed that the ATF4 ODD domain induced HSV-tk expression under hypoxia and facilitated the death of C6 cells in the presence of ganciclovir (GCV). Furthermore, pSV-HSVtk-ATF4-ODD induced caspase-3 activity in the hypoxic cells. In conclusion, the ATF4 ODD may be useful for hypoxia-specific gene therapy by post-translational regulation of gene expression.en_US
dc.description.sponsorshipThis work was financially supported by a grant from the National Research Foundation of Korea, funded by the Ministry of Education, Science and Technology (20110027482 and 2012K001394).en_US
dc.language.isoenen_US
dc.publisherInforma Healthcareen_US
dc.subjectActivating transcription factor-4en_US
dc.subjectgene regulationen_US
dc.subjectgene therapyen_US
dc.subjecthypoxiaen_US
dc.subjectpost-translational regulationen_US
dc.titlePost-translational regulation of gene expression using the ATF4 oxygen-dependent degradation domain for hypoxia-specific gene therapyen_US
dc.typeArticleen_US
dc.relation.volume21-
dc.identifier.doi10.3109/1061186X.2013.829073-
dc.relation.page830-836-
dc.relation.journalJOURNAL OF DRUG TARGETING-
dc.contributor.googleauthorCho, Su Hee-
dc.contributor.googleauthorOh, Binna-
dc.contributor.googleauthorKim, Hyun Ah-
dc.contributor.googleauthorPark, Jeong Hyun-
dc.contributor.googleauthorLee, Minhyung-
dc.relation.code2013010630-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidminhyung-
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE