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Evaluation of transdermal delivery of nanoemulsions in ex vivo porcine skin using two-photon microscopy and confocal laser-scanning microscopy

Title
Evaluation of transdermal delivery of nanoemulsions in ex vivo porcine skin using two-photon microscopy and confocal laser-scanning microscopy
Author
김진웅
Keywords
transdermal delivery; two-photon microscopy; two-photon microscopy; confocal laser scanning microscopy
Issue Date
2014-10
Publisher
SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
Citation
JOURNAL OF BIOMEDICAL OPTICS, 권: 19, 호: 10
Abstract
This study experimentally evaluates the self-targeting ability of asiaticoside-loaded nanoemulsions compared with nontargeted nanoemulsions in ex vivo experiments with porcine skin samples. Homebuilt two-photon and confocal laser-scanning microscopes were employed to noninvasively examine the transdermal delivery of two distinct nanoemulsions. Prior to the application of nanoemulsions, we noninvasively observed the morphology of porcine skin using two-photon microscopy. We have successfully visualized the distributions of the targeted and nontargeted nanoemulsions absorbed into the porcine skin samples. Asiaticoside-loaded nanoemulsions showed an improved ex vivo transdermal delivery through the stratum corneum compared with nonloaded nanoemulsions. As a secondary measure, nanoemulsions-applied samples were sliced in the depth direction with a surgical knife in order to obtain the complete depth-direction distribution profile of Nile red fluorescence. XZ images demonstrated that asiaticoside-loaded nanoemulsion penetrated deeper into the skin compared with nontargeted nanoemulsions. The basal layer boundary is clearly visible in the case of the asiaticoside-loaded skin sample. These results reaffirm the feasibility of using self-targeting ligands to improve permeation through the skin barrier for cosmetics and topical drug applications. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
URI
http://www.spiedigitallibrary.org/journals/Journal_of_Biomedical_Optics/volume-19/issue-10/106006/Evaluation-of-transdermal-delivery-of-nanoemulsions-in-ex-vivo-porcine/10.1117/1.JBO.19.10.106006.fullhttp://hdl.handle.net/20.500.11754/50688
ISSN
1083-3668; 1560-2281
DOI
10.1117/1.JBO.19.10.10600610.1117/1.JBO.19.10.106006
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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