Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 류재숙 | - |
dc.date.accessioned | 2018-03-21T06:23:44Z | - |
dc.date.available | 2018-03-21T06:23:44Z | - |
dc.date.issued | 2014-04 | - |
dc.identifier.citation | PROSTATE; APR 2014, 74, 4, p441-p449 | en_US |
dc.identifier.issn | 0270-4137 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/abs/10.1002/pros.22766 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/50048 | - |
dc.description.abstract | BACKGROUND Trichomonas vaginalis is known as the most common cause of sexually transmitted infection. However, its prevalence may have been underestimated. Trichomonads are detected in prostatic tissue in benign prostatic hyperplasia, prostatitis, and prostate cancer. Our objective was to investigate whether T. vaginalis could induce an inflammatory response in prostate epithelium.METHODSThe cytokine production by human prostate epithelial cell (RWPE-1) activated with T. vaginalis was determined by ELISA and real-time PCR. Intracellular ROS was evaluated by flow cytometry or spectrofluorometry. The protein levels of MAP kinase, NF-B were analyzed by Western blot. The migration of neutrophil and monocyte were performed in 24-well microplates with filter insert.RESULTSIncubation of cells of a human prostate epithelial cell line with a live T. vaginalis T016 isolate increased expression of the inflammatory mediators IL-1, CCL2, and CXCL8. In addition, ROS, MAPK, and NF-B activities increased, while inhibitors of ROS, ERK, and NF-B reduced IL-1 production. Medium conditioned by incubation of RWPE-1 cells with T. vaginalis contained IL-1 and stimulated the migration of human neutrophils and monocytes (THP-1 cell line).CONCLUSIONSWe conclude that T. vaginalis may increase IL-1 expression in human prostate epithelium through activation of ROS, ERK, and NF-B, and this in turn may induce the migration of neutrophils and monocytes and lead to an inflammatory response. This research is the first attempt to confirm inflammatory reaction caused by T. vaginalis in prostate epithelial cell. Prostate 74:441-449, 2014. (c) 2013 Wiley Periodicals, Inc. | en_US |
dc.description.sponsorship | This research was supported by the Basic ScienceResearch Program through the National ResearchFoundation of Korea (NRF) funded by the Ministry ofEducation, Science and Technology (2011-0012374). | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA | en_US |
dc.subject | prostate epithelial cell | en_US |
dc.subject | Trichomonas vaginalis | en_US |
dc.subject | IL-1 | en_US |
dc.subject | neutrophil | en_US |
dc.subject | monocyte migration | en_US |
dc.subject | SEXUALLY-TRANSMITTED-DISEASES | en_US |
dc.subject | SIGNAL-REGULATED KINASE | en_US |
dc.subject | TRICHOMONAS-VAGINALIS | en_US |
dc.subject | HUMAN NEUTROPHILS | en_US |
dc.subject | MAST-CELLS | en_US |
dc.subject | CHLAMYDIA-TRACHOMATIS | en_US |
dc.subject | INFECTION | en_US |
dc.subject | PREVALENCE | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | APOPTOSIS | en_US |
dc.title | Inflammatory Response of Prostate Epithelial Cells to Stimulation byTrichomonas vaginalis | en_US |
dc.type | Article | en_US |
dc.relation.volume | 74 | - |
dc.identifier.doi | 10.1002/pros.22766 | - |
dc.relation.page | 441-449 | - |
dc.relation.journal | PROSTATE | - |
dc.contributor.googleauthor | Seo, Min-Young | - |
dc.contributor.googleauthor | Im, Su-Jeong | - |
dc.contributor.googleauthor | Gu, Na-Young | - |
dc.contributor.googleauthor | Kim, Jung-Hyun | - |
dc.contributor.googleauthor | Ahn, Myoung-Hee | - |
dc.contributor.googleauthor | Ryu, Jae-Sook | - |
dc.contributor.googleauthor | Chung, Yong-Hoon | - |
dc.relation.code | 2014038184 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jsryu | - |
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