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Protective effect of a freshwater alga, Spirogyra sp., against lipid peroxidation in vivo zebrafish and purification of antioxidative compounds using preparative centrifugal partition chromatography

Title
Protective effect of a freshwater alga, Spirogyra sp., against lipid peroxidation in vivo zebrafish and purification of antioxidative compounds using preparative centrifugal partition chromatography
Author
김철영
Keywords
Spirogyra sp.; Antilipid peroxidation; ABTS(+) online HPLC; Centrifugal partition chromatography (CPC); Zebrafish
Issue Date
2016-02
Publisher
SPRINGER
Citation
JOURNAL OF APPLIED PHYCOLOGY, v. 28, No. 1, Page. 181-189
Abstract
A freshwater alga, Spirogyra sp., collected in shallow ponds in South Korea, was evaluated for its antilipid peroxidative effect against 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced in vivo zebrafish, and antioxidative compounds from the alga were efficiently identified using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS(+)) online high-performance liquid chromatography (HPLC) and preparative centrifugal partition chromatography. The ethyl acetate fraction of Spirogyra sp. (SPE) in each fraction showed the strongest 2,2-diphenyl-1-picrylhydrazyl scavenging activity and significantly scavenged 2',7'-dichlorodihydrofluorescein diacetate and diphenyl-1-pyrenylphosphine fluorescence, respectively, in AAPH-induced zebrafish embryo without any cytotoxicity in the concentrations between 25 and 50 mu g mL(-1). The two main antioxidative compounds in SPE were confirmed by ABTS(+) online HPLC and were identified as gallic acid and methyl gallate, respectively, by HPLC-diode array detection (DAD)-electrospray ionization (ESI)/mass spectrometry (MS), H-1- and C-13-NMR. We conclude therefore that Spirogyra sp. is rich in gallic acid and methyl gallate, and it might be useful as a strong antilipid peroxidation material.
URI
https://link.springer.com/article/10.1007/s10811-015-0548-yhttp://hdl.handle.net/20.500.11754/49806
ISSN
0921-8971; 1573-5176
DOI
10.1007/s10811-015-0548-y
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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