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HPLC Determination of Bioactive Flavonoids in Hovenia dulcis Fruit Extracts

Title
HPLC Determination of Bioactive Flavonoids in Hovenia dulcis Fruit Extracts
Author
유혜현
Keywords
SEMEN-SEU-FRUCTUS; ABSOLUTE STEREOSTRUCTURES; TRITERPENE GLYCOSIDES; ANTIOXIDANT ACTIVITY; THUNB; QUERCETIN; MYRICETIN; DIHYDROMYRICETIN; SEEDS; MICE
Issue Date
2016-02
Publisher
OXFORD UNIV PRESS INC
Citation
JOURNAL OF CHROMATOGRAPHIC SCIENCE, v. 54, No. 2, Page. 130-135
Abstract
A simple, accurate and reproducible reversed-phase liquid chromatographic method was developed for qualitative and quantitative determination of four bioactive flavonoids (ampelopsin, taxifolin, myricetin and quercetin) from the fruit-stalk extract of Hovenia dulcis Thunb. Chromatographic separation was performed on a C18 column (4.6 x 150 mm, 3.5 mu m) with mobile phase consisting of 0.1% acetic acid and 100% acetonitrile at a flow rate of 1.0 mL/min. The analysis was performed using a diode array detector at 365 nm. The method was validated in terms of selectivity, linearity, accuracy, precision and recovery. Good linearity was observed over the investigated concentration range (10-500 mu g/mL), with correlation coefficient values greater than 0.99. The intra-and inter-day precisions over the concentration range were ˂3.91% (relative standard deviation), and the accuracy was between 91.57 and 106.66%. The mean recovery for all the analytes was 100.87%. This method was successfully applied in the quality assessment of bioactive flavonoids in the fruit-stalk extract of H. dulcis.
URI
https://academic.oup.com/chromsci/article/54/2/130/2754733http://hdl.handle.net/20.500.11754/49767
ISSN
0021-9665; 1945-239X
DOI
10.1093/chromsci/bmv114
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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