Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 유혜현 | - |
dc.date.accessioned | 2018-03-20T07:40:03Z | - |
dc.date.available | 2018-03-20T07:40:03Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.citation | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v. 120, Page. 19-24 | en_US |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.issn | 1873-264X | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0731708515302685 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/49763 | - |
dc.description.abstract | Kinsenoside is a major bioactive constituent isolated from Anoectochilus formosanus and is investigated as an antihyperlipidemic candidate. In this study, a rapid, sensitive, and reliable bioanalytical method was developed for the determination of kinsenoside in rat plasma using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The plasma sample was pretreated with 1% acetic acid, followed by protein precipitation with acetonitrile:methanol (70:30). Chromatographic separation was performed on a HILIC silica column (2.1 mm x 100 mm, 3 mu m). The mobile phases consisted of 0.1% acetic acid in distilled water (solvent A) and 0.1% acetic acid in acetonitrile (solvent B). A gradient program was used at a flow rate of 0.2 mi./min. For mass spectrometric detection, the multiple reaction monitoring mode was used; the MRM transitions were m/z 265.2 -> m/z 102.9 for kinsenoside and m/z 1633 -> m/z 132.1 for the internal standard (IS) nicotine in the positive ionization mode. A calibration curve was constructed in the range of 2-500 ng/mL. The intra- and interday precision and accuracy were within 5%. The HILIC-MS/MS method was specific, accurate, and reproducible and was successfully applied in a pharmacokinetic study of kinsenoside in rats. (C) 2015 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (NRF-2012K1A3A1A20031104). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Kinsenoside | en_US |
dc.subject | Antihyperlipidemic agent | en_US |
dc.subject | LC-MS/MS | en_US |
dc.subject | Plasma | en_US |
dc.subject | Pharmacokinetics | en_US |
dc.title | Development of a hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the determination of kinsenoside, an antihyperlipidemic candidate, in rat plasma and its application to pharmacokinetic studies | en_US |
dc.type | Article | en_US |
dc.relation.volume | 120 | - |
dc.identifier.doi | 10.1016/j.jpba.2015.12.003 | - |
dc.relation.page | 19-24 | - |
dc.relation.journal | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | - |
dc.contributor.googleauthor | Rehman, Shaheed Ur | - |
dc.contributor.googleauthor | Kim, In Sook | - |
dc.contributor.googleauthor | Choi, Min Sun | - |
dc.contributor.googleauthor | Luo, Zengwei | - |
dc.contributor.googleauthor | Yao, Guangming | - |
dc.contributor.googleauthor | Xue, Yongbo | - |
dc.contributor.googleauthor | Zhang, Yonghui | - |
dc.contributor.googleauthor | Yoo, Hye Hyun | - |
dc.relation.code | 2016000071 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | yoohh | - |
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