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Fluoxetine-induced regulation of heat shock protein 90 and 14-3-3 epsilon in human embryonic carcinoma cells

Title
Fluoxetine-induced regulation of heat shock protein 90 and 14-3-3 epsilon in human embryonic carcinoma cells
Author
최준호
Keywords
antidepressive agents; cell line; molecular chaperones; proteomics
Issue Date
2014-12
Publisher
Lippincott Williams & Wilkins
Citation
Neuro Report, 2014, 26(17), P.1399-1404
Abstract
Fluoxetine, a serotonin-selective reuptake inhibitor, exerts antidepressant and antianxiety effects on major depressive and anxiety disorders. Previous studies suggest that treatment with fluoxetine influences the expression of various proteins that are involved in proliferation, differentiation, and apoptosis in the neuronal cells of the brain. However, many aspects of the molecular pathways that modulate antidepressant action are not well understood. Here, with the aim of identifying proteins involved in antidepressant action, we examined the protein expression profile of human embryonic carcinoma (NCCIT) cells in response to fluoxetine treatment using proteomic techniques such as two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). We found several upregulated and downregulated proteins in fluoxetine-treated NCCIT cells, and then biochemically confirmed the increased expression of heat shock protein 90 and 14-3-3[epsilon], which play an essential role in many cellular mechanisms including cell cycle control and other signaling pathways. Our data suggest that the regulated expression of heat shock protein 90, 14-3-3[epsilon], and other identified proteins may be associated with the therapeutic action of fluoxetine.
URI
https://journals.lww.com/neuroreport/Abstract/2014/12030/Fluoxetine_induced_regulation_of_heat_shock.12.aspx
ISSN
0959-4965
DOI
10.1097/WNR.0000000000000284
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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