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dc.contributor.author채영규-
dc.date.accessioned2018-03-20T00:59:36Z-
dc.date.available2018-03-20T00:59:36Z-
dc.date.issued2013-03-
dc.identifier.citationBIOCHIP JOURNAL, 2013, 7(1), p.75-84en_US
dc.identifier.issn1976-0280-
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs13206-013-7112-0-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/49207-
dc.description.abstractAnthrax lethal toxin (LeTx; a combination of protective antigen and lethal factor) is secreted by the vegetative cells of Bacillus anthracis and is cytotoxic for certain macrophage cell lines. First-time exposure of murine macrophage cells (RAW 264.7) to lethal toxin (LeTx) (0.1+0.1 mg/mL) caused extensive cell death with a survival rate of approximately 40%, but upon secondary exposure to LeTx and lipopolysaccharide (LPS) (1 mu g/mL), after a few passages, these cells had a survival rate of approximately 100%. The present study assessed protein expression changes after LPS exposure to LeTx-intoxication-resistant RAW 264.7 cells. To analyze the protein expression profile of LPS-treated LeTx-intoxication-resistant RAW 264.7 cells, we employed matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDITOF MS), and later, Ingenuity Pathways Analysis (IPA) was applied to establish the molecular networks. Among the differentially expressed proteins were voltage dependent anion channel 1, jip3 protein, heat shock protein 4, tubulin beta, 26S protease regulatory subunit 4, and DNA polymerase delta subunit 4 (DNA pol delta) were significant. The molecular network signatures and functional proteomics obtained in this study may facilitate the evaluation of potential pathways such as PI3K signaling, NF-kappa B signaling, and LPSinduced MAPK signaling for the recovery of LPSinduced LeTx-intoxication-resistant RAW 264.7 cells.en_US
dc.description.sponsorshipThis study was supported by Science and Technology Research Grant number HY-2010-N from Hanyang University, Korea.en_US
dc.language.isoenen_US
dc.publisherKOREAN BIOCHIP SOCIETY-KBCS, KOREA SCI & TECHNOL CENT, #310, 635-4, YEOGSAM-DONG, KANGNAM-GU, SEOUL, 135-703, SOUTH KOREAen_US
dc.subjectAnthrax lethal toxin (LeTx)en_US
dc.subjectCell survivalen_US
dc.subjectIngenuity Pathway Analysis (IPA)en_US
dc.subjectLipopolysaccharides (LPS)en_US
dc.subjectLeTx-intoxication-resistant RAW 264.7 cellen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectDNA-POLYMERASE-DELTAen_US
dc.subjectPROTEIN-DISULFIDE-ISOMERASEen_US
dc.subjectPERICENTROMERIC HETEROCHROMATINen_US
dc.subjectSIGNALING PATHWAYen_US
dc.subjectMOLECULAR-CLONINGen_US
dc.subjectPEPTIDASE-Ien_US
dc.subjectKAPPA-Ben_US
dc.subjectMACROPHAGESen_US
dc.subjectAPOPTOSISen_US
dc.subjectSUBUNITen_US
dc.titleIdentification of survival factors in LPS-stimulated anthrax lethal toxin tolerant RAW 264.7 cells through proteomic approachen_US
dc.typeArticleen_US
dc.relation.volume7-
dc.identifier.doi10.1007/s13206-013-7112-0-
dc.relation.page75-84-
dc.relation.journalBIOCHIP JOURNAL-
dc.contributor.googleauthorDas, Amitabh-
dc.contributor.googleauthorDas, Nando Dulal-
dc.contributor.googleauthorPark, JiHyun-
dc.contributor.googleauthorLee, HyungTae-
dc.contributor.googleauthorChoi, MiRan-
dc.contributor.googleauthorJung, KyoungHwa-
dc.contributor.googleauthorChai, YoungGyu-
dc.relation.code2013001127-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidygchai-
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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