Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells

Title
Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells
Author
류재숙
Keywords
Trichomonas vaginalis; 1,10-phenanthroline; mTOR cleavage; SiHa cell; metalloproteinase
Issue Date
2014-12
Publisher
Korean SOC Parasitology
Citation
Korean Journal of Parasitology, 2014, 52(6), P.595-603
Abstract
Trichomonas vaginallis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T vaginas trophozoites, and T vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T vaginas, T. vaginalis excretory-secretory products (ESP) or T vaginalis lysate, live T vaginalis and T vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T vaginalis lysate did not. Pretreatment of T vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T vaginas metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite.
URI
http://parasitol.kr/journal/view.php?doi=10.3347/kjp.2014.52.6.595http://hdl.handle.net/20.500.11754/49191
ISSN
0023-4001; 1738-0006
DOI
10.3347/kjp.2014.52.6.595
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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