Butyl paraben-induced changes in DNA methylation in rat epididymal spermatozoa

Title
Butyl paraben-induced changes in DNA methylation in rat epididymal spermatozoa
Authors
박찬진
Keywords
Butyl paraben; DNA methylation; epididymal spermatozoa; rat
Issue Date
2012-05
Publisher
Blackwell Publishing Ltd
Citation
Andrologia, 2012, 44(1), P.187-193
Abstract
Parabens have been shown to affect male rodent reproductive parameters, including testosterone levels and sperm production. In this study, we examined the effect of long-term exposure to butyl paraben (BP) on rat epididymal sperm DNA methylation. Adult male rats were exposed to BP (0, 10, 100 and 1000 mg kg-1 per day) according to OECD TG407 for a repeated 28-day oral toxicity study. Sperm DNA methylation was examined by differential display random amplification of polymorphic DNA (RAPD) following methylation-specific restriction digestion of DNA. Among the 57 RAPD amplicons, six were methylation specific. Of these, five amplicons increased by 1.4- to 3.8-fold in epididymal sperm DNA at testing dose of BP. This indicates that BP can cause DNA hypermethylation in germ cells from the mitotic through post-meiotic stage in adult rat testes. To our knowledge, this is the first report on the epigenetic modification of sperm DNA by parabens.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0272.2011.01162.xhttp://hdl.handle.net/20.500.11754/49098
ISSN
0303-4569
DOI
http://dx.doi.org/10.1111/j.1439-0272.2011.01162.x
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RESEARCH INSTITUTE[S](부설연구소) > THE RESEARCH INSTITUTE FOR NATURAL SCIENCES(자연과학연구소) > Articles
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