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Semi-quantitative Estimation of Cellular SiO2 Nanoparticles Using Flow Cytometry Combined with X-ray Fluorescence Measurements

Title
Semi-quantitative Estimation of Cellular SiO2 Nanoparticles Using Flow Cytometry Combined with X-ray Fluorescence Measurements
Author
윤태현
Keywords
flow cytometry; cellular SiO2 nanoparticles; X-ray fluorescence; nanoparticle toxicity
Issue Date
2014-09
Publisher
WILEY-BLACKWELL
Citation
CYTOMETRY PART A, SEP 2014, 85(9), p771-p780, 10p.
Abstract
In this study, we have demonstrated feasibility of a semi-quantitative approach for the estimation of cellular SiO2 nanoparticles (NPs), which is based on the flow cytometry measurements of their normalized side scattering intensity. In order to improve our understanding on the quantitative aspects of cell-nanoparticle interactions, flow cytometry, transmission electron microscopy, and X-ray fluorescence experiments were carefully performed for the HeLa cells exposed to SiO2 NPs with different core diameters, hydrodynamic sizes, and surface charges. Based on the observed relationships among the experimental data, a semi-quantitative cellular SiO2 NPs estimation method from their normalized side scattering and core diameters was proposed, which can be applied for the determination of cellular SiO2 NPs within their size-dependent linear ranges. (C) 2014 International Society for Advancement of Cytometry
URI
http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22481/abstract;jsessionid=E4AFAD2EE505AA8E33056677C8C2A06C.f03t03http://hdl.handle.net/20.500.11754/48509
ISSN
1552-4922; 1552-4930
DOI
10.1002/cyto.a.22481
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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