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Hydrophobic variants of ganglio-tripod amphiphiles for membrane protein manipulation

Title
Hydrophobic variants of ganglio-tripod amphiphiles for membrane protein manipulation
Author
채필석
Keywords
Amphiphile; Membrane protein; Molecular design; Protein solubilization; Membrane mimetic
Issue Date
2014-01
Publisher
Elsevier B.V.
Citation
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2014, 1838(1), Part B, P.278-286
Abstract
Membrane proteins operate in unique cellular environments. Once removed from their native context for the purification that is required for most types of structural or functional analyses, they are prone to denature if not properly stabilized by membrane mimetics. Detergent micelles have prominently been used to stabilize membrane proteins in aqueous environments as their amphipathic nature allows for shielding of the hydrophobic surfaces of these bio-macromolecules while supporting solubility and monodispersity in water. This study expands the utility of branched diglucoside-bearing tripod agents, designated ganglio-tripod amphiphiles, with introduction of key variations in their hydrophobic sections and shows how these latter elements can be fine-tuned to maximize membrane protein solubilization while preserving characteristics of these molecules that afford stabilization of rather fragile assemblies. Their efficacy rivals benchmark detergents heavily used today, such as n-dodecyl-β-d-maltoside.
URI
https://www.sciencedirect.com/science/article/pii/S0005273613003167http://hdl.handle.net/20.500.11754/47516
ISSN
0005-2736
DOI
10.1016/j.bbamem.2013.09.011
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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